Cell type- and brain structure-specific patterns of distribution of minibrain kinase in human brain

Jerzy Wegiel, Izabela Kuchna, Krzysztof Nowicki, Janusz Frackowiak, Karol Dowjat, Wayne P Silverman, Barry Reisberg, Mony Deleon, Thomas Wisniewski, Tatyana Adayev, Mo Chou Chen-Hwang, Yu Wen Hwang

Research output: Contribution to journalArticle

Abstract

The minibrain kinase (Mnb/Dyrk1A) gene is localized in the Down syndrome (DS) critical region of chromosome 21. This gene encodes a proline-directed serine/threonine protein kinase (minibrain kinase-Mnb/Dyrk1A), which is required for the proliferation of distinct neuronal cell types during postembryonic neurogenesis. To study the distribution of Mnb/Dyrk1A during human brain development and aging, we raised Mnb/Dyrk1A-specific antibody (mAb 7F3) and examined 22 brains of normal subjects from 8 months to 90 years of age. We found that neurons were the only cells showing the presence of 7F3-positive product in both cell nucleus and cytoplasm. Nuclear localization supports the concept that Mnb/Dyrk1A may be involved in control of gene expression. Synaptic localization of Mnb/Dyrk1A also supports our previous studies suggesting that Mnb/Dyrk1A is a regulator of assembly of endocytic apparatus and appears to be involved in synaptic vesicle recycling and synaptic signal transmission. Accumulation of numerous 7F3-positive corpora amylacea in the memory and motor system subdivisions in subjects older than 33 years of age indicates that Mnb/Dyrk1A is colocalized with markers of astrocyte and neuron degeneration. Differences in the topography and the amount of Mnb/Dyrk1A in neurons, astrocytes, and ependymal and endothelial cells appear to reflect cell type- and brain structure-specific patterns in trafficking and utilization of Mnb/Dyrk1A.

Original languageEnglish (US)
Pages (from-to)69-80
Number of pages12
JournalBrain Research
Volume1010
Issue number1-2
DOIs
StatePublished - Jun 4 2004
Externally publishedYes

Fingerprint

Astrocytes
Proline-Directed Protein Kinases
Brain
Phosphotransferases
Neurons
Chromosomes, Human, Pair 21
Nerve Degeneration
Synaptic Vesicles
Protein-Serine-Threonine Kinases
Neurogenesis
Human Development
Cell Nucleus
Synaptic Transmission
Genes
Cytoplasm
Endothelial Cells
Gene Expression
Antibodies
Dyrk kinase
Down Syndrome Critical Region

Keywords

  • Brain aging
  • Brain development
  • Cellular and molecular biology
  • Corpora amylacea
  • Immunocytochemistry
  • Minibrain kinase
  • Staining, tracing and imaging techniques
  • Synapse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Wegiel, J., Kuchna, I., Nowicki, K., Frackowiak, J., Dowjat, K., Silverman, W. P., ... Hwang, Y. W. (2004). Cell type- and brain structure-specific patterns of distribution of minibrain kinase in human brain. Brain Research, 1010(1-2), 69-80. https://doi.org/10.1016/j.brainres.2004.03.008

Cell type- and brain structure-specific patterns of distribution of minibrain kinase in human brain. / Wegiel, Jerzy; Kuchna, Izabela; Nowicki, Krzysztof; Frackowiak, Janusz; Dowjat, Karol; Silverman, Wayne P; Reisberg, Barry; Deleon, Mony; Wisniewski, Thomas; Adayev, Tatyana; Chen-Hwang, Mo Chou; Hwang, Yu Wen.

In: Brain Research, Vol. 1010, No. 1-2, 04.06.2004, p. 69-80.

Research output: Contribution to journalArticle

Wegiel, J, Kuchna, I, Nowicki, K, Frackowiak, J, Dowjat, K, Silverman, WP, Reisberg, B, Deleon, M, Wisniewski, T, Adayev, T, Chen-Hwang, MC & Hwang, YW 2004, 'Cell type- and brain structure-specific patterns of distribution of minibrain kinase in human brain', Brain Research, vol. 1010, no. 1-2, pp. 69-80. https://doi.org/10.1016/j.brainres.2004.03.008
Wegiel J, Kuchna I, Nowicki K, Frackowiak J, Dowjat K, Silverman WP et al. Cell type- and brain structure-specific patterns of distribution of minibrain kinase in human brain. Brain Research. 2004 Jun 4;1010(1-2):69-80. https://doi.org/10.1016/j.brainres.2004.03.008
Wegiel, Jerzy ; Kuchna, Izabela ; Nowicki, Krzysztof ; Frackowiak, Janusz ; Dowjat, Karol ; Silverman, Wayne P ; Reisberg, Barry ; Deleon, Mony ; Wisniewski, Thomas ; Adayev, Tatyana ; Chen-Hwang, Mo Chou ; Hwang, Yu Wen. / Cell type- and brain structure-specific patterns of distribution of minibrain kinase in human brain. In: Brain Research. 2004 ; Vol. 1010, No. 1-2. pp. 69-80.
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