Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography

Jun Liu, Mark J. McBride, Sriram Subramaniam

Research output: Contribution to journalArticle

Abstract

Flavobacterium johnsoniae cells glide rapidly over surfaces by an as-yet-unknown mechanism. Using cryo-electron tomography, we show that wild-type cells display tufts of ∼5-nm-wide cell surface filaments that appear to be anchored to the inner surface of the outer membrane. These filaments are absent in cells of a nonmotile gldF mutant but are restored upon expression of plasmid-encoded GldF, a component of a putative ATP-binding cassette transporter.

Original languageEnglish (US)
Pages (from-to)7503-7506
Number of pages4
JournalJournal of Bacteriology
Volume189
Issue number20
DOIs
StatePublished - Oct 2007
Externally publishedYes

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Electron Microscope Tomography
Flavobacterium
Bacteria
ATP-Binding Cassette Transporters
Plasmids
Membranes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography. / Liu, Jun; McBride, Mark J.; Subramaniam, Sriram.

In: Journal of Bacteriology, Vol. 189, No. 20, 10.2007, p. 7503-7506.

Research output: Contribution to journalArticle

Liu, Jun ; McBride, Mark J. ; Subramaniam, Sriram. / Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 20. pp. 7503-7506.
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