Cell surface-associated growth inhibitory proteins. Evidence for conservation between mouse and human cell lines

Michael A. Lieberman, Dan Raben, Luis Glaser

Research output: Contribution to journalArticle

Abstract

The ability of the normal human fibroblast line (IMR91) to exhibit density-dependent regulation of growth has been examined. The line exhibits density-dependent regulation of growth; saturation density in 15% fetal bovine serum is 2 × 105 cells/cm2. Membranes prepared from confluent monolayers of these cells contained growth inhibitory factors to both exponentially growing IMR91 and Swiss 3T3 cells. This factor(s) appears to be similar to a previously described factor found on the surface of Swiss 3T3 cells [14]. The inhibition of DNA synthesis in growing IMR91 cultures by membranes was both time- and concentration-dependent. The effect was reversible by high serum. Specificity experiments utilizing membranes prepared from Swiss 3T3 cells indicated some species specificity for inhibition by membranes, but this specificity was no longer exhibited by solubilized membrane preparations. These results are compatible with the suggestion that both the growth inhibitory factors and their receptors are conserved through evolution.

Original languageEnglish (US)
Pages (from-to)413-419
Number of pages7
JournalExperimental cell research
Volume133
Issue number2
DOIs
StatePublished - Jun 1981
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology

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