Cell surface antigens of a mouse testicular teratoma. Identification of an antigen physically associated with H 2 antigens on tumor cells

L. R. Gooding, Michael A Edidin

Research output: Contribution to journalArticle

Abstract

Rabbit antisera to a mouse testicular teratoma absorbed with normal mouse tissues, react by immunofluorescence with plasma membrane antigens of a variety of transplantable mouse tumor cells and transformed fibroblast cell lines including Clone 1D, SV 40 3T3, and 3T12. Trypsin treatment of cells of 'normal' lines, 3T3 and FR SV 3T3, uncovers reactivity on these as well. Early passage mouse embryo fibroblast cell cultures do not react even after trypsinization. By cross absorption studies, the anti teratoma serum appears to react with an antigen common to most tumor cells investigated thus far. When this antigen on Clone 1D cells is 'capped', H 2 antigens collect with the teratoma antigens in the cap indicating a physical association between the molecules. Molecules specified by both the H 2D and H 2K regions are bound to the teratoma antigens in the Clone 1D plasma membrane. This antigen is also found in soluble tumor cell fractions where it is believed to be free of H 2. A second cell surface antigen defined by anti teratoma serum is expressed only by hepatoma and teratoma itself. This second antigen is apparently a secretory product of teratoma cells. A third surface antigen defined by anti teratoma serum appears to be specific for the teratoma.

Original languageEnglish (US)
Pages (from-to)61-78
Number of pages18
JournalJournal of Experimental Medicine
Volume140
Issue number1
StatePublished - 1974

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H-2 Antigens
Teratoma
Surface Antigens
Antigens
Neoplasms
Clone Cells
Fibroblasts
Serum
Cell Membrane
Transformed Cell Line
Testicular Teratoma
Trypsin
Fluorescent Antibody Technique
Immune Sera
Hepatocellular Carcinoma
Embryonic Structures
Cell Culture Techniques
Rabbits
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Cell surface antigens of a mouse testicular teratoma. Identification of an antigen physically associated with H 2 antigens on tumor cells",
abstract = "Rabbit antisera to a mouse testicular teratoma absorbed with normal mouse tissues, react by immunofluorescence with plasma membrane antigens of a variety of transplantable mouse tumor cells and transformed fibroblast cell lines including Clone 1D, SV 40 3T3, and 3T12. Trypsin treatment of cells of 'normal' lines, 3T3 and FR SV 3T3, uncovers reactivity on these as well. Early passage mouse embryo fibroblast cell cultures do not react even after trypsinization. By cross absorption studies, the anti teratoma serum appears to react with an antigen common to most tumor cells investigated thus far. When this antigen on Clone 1D cells is 'capped', H 2 antigens collect with the teratoma antigens in the cap indicating a physical association between the molecules. Molecules specified by both the H 2D and H 2K regions are bound to the teratoma antigens in the Clone 1D plasma membrane. This antigen is also found in soluble tumor cell fractions where it is believed to be free of H 2. A second cell surface antigen defined by anti teratoma serum is expressed only by hepatoma and teratoma itself. This second antigen is apparently a secretory product of teratoma cells. A third surface antigen defined by anti teratoma serum appears to be specific for the teratoma.",
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N2 - Rabbit antisera to a mouse testicular teratoma absorbed with normal mouse tissues, react by immunofluorescence with plasma membrane antigens of a variety of transplantable mouse tumor cells and transformed fibroblast cell lines including Clone 1D, SV 40 3T3, and 3T12. Trypsin treatment of cells of 'normal' lines, 3T3 and FR SV 3T3, uncovers reactivity on these as well. Early passage mouse embryo fibroblast cell cultures do not react even after trypsinization. By cross absorption studies, the anti teratoma serum appears to react with an antigen common to most tumor cells investigated thus far. When this antigen on Clone 1D cells is 'capped', H 2 antigens collect with the teratoma antigens in the cap indicating a physical association between the molecules. Molecules specified by both the H 2D and H 2K regions are bound to the teratoma antigens in the Clone 1D plasma membrane. This antigen is also found in soluble tumor cell fractions where it is believed to be free of H 2. A second cell surface antigen defined by anti teratoma serum is expressed only by hepatoma and teratoma itself. This second antigen is apparently a secretory product of teratoma cells. A third surface antigen defined by anti teratoma serum appears to be specific for the teratoma.

AB - Rabbit antisera to a mouse testicular teratoma absorbed with normal mouse tissues, react by immunofluorescence with plasma membrane antigens of a variety of transplantable mouse tumor cells and transformed fibroblast cell lines including Clone 1D, SV 40 3T3, and 3T12. Trypsin treatment of cells of 'normal' lines, 3T3 and FR SV 3T3, uncovers reactivity on these as well. Early passage mouse embryo fibroblast cell cultures do not react even after trypsinization. By cross absorption studies, the anti teratoma serum appears to react with an antigen common to most tumor cells investigated thus far. When this antigen on Clone 1D cells is 'capped', H 2 antigens collect with the teratoma antigens in the cap indicating a physical association between the molecules. Molecules specified by both the H 2D and H 2K regions are bound to the teratoma antigens in the Clone 1D plasma membrane. This antigen is also found in soluble tumor cell fractions where it is believed to be free of H 2. A second cell surface antigen defined by anti teratoma serum is expressed only by hepatoma and teratoma itself. This second antigen is apparently a secretory product of teratoma cells. A third surface antigen defined by anti teratoma serum appears to be specific for the teratoma.

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