Cell-specific mitotic defect and dyserythropoiesis associated with erythroid band 3 deficiency

Barry H. Paw, Alan J. Davidson, Yi Zhou, Rong Li, Stephen J. Pratt, Charles Lee, Nikolaus S. Trede, Alison Brownlie, Adriana Donovan, Eric C. Liao, James M. Ziai, Anna H. Drejer, Wen Guo, Carol H. Kim, Babette Gwynn, Luanne L. Peters, Marina N. Chernova, Seth L. Alper, Agustin Zapata, Sunitha N. WickramasingheMatthew J. Lee, Samuel E. Lux, Andreas Fritz, John H. Postlethwait, Leonard I. Zon

Research output: Contribution to journalArticle

Abstract

Most eukaryotic cell types use a common program to regulate the process of cell division. During mitosis, successful partitioning of the genetic material depends on spatially coordinated chromosome movement and cell cleavage. Here we characterize a zebrafish mutant, retsina (ret), that exhibits an erythroid-specific defect in cell division with marked dyserythropoiesis similar to human congenital dyserythropoietic anemia. Erythroblasts from retfish show binuclearity and undergo apoptosis due to a failure in the completion of chromosome segregation and cytokinesis. Through positional cloning, we show that the ret mutation is in a gene (slc4a1) encoding the anion exchanger 1 (also called band 3 and AE1), an erythroid-specific cytoskeletal protein. We further show an association between deficiency in Slc4a1 and mitotic defects in the mouse. Rescue experiments in ret zebrafish embryos expressing transgenic slc4a1 with a variety of mutations show that the requirement for band 3 in normal erythroid mitosis is mediated through its protein 4.1R-binding domains. Our report establishes an evolutionarily conserved role for band 3 in erythroid-specific cell division and illustrates the concept of cell-specific adaptation for mitosis.

Original languageEnglish (US)
Pages (from-to)59-64
Number of pages6
JournalNature Genetics
Volume34
Issue number1
DOIs
StatePublished - May 1 2003
Externally publishedYes

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Mitosis
Cell Division
Zebrafish
Congenital Dyserythropoietic Anemia
Erythrocyte Anion Exchange Protein 1
Erythroblasts
Chromosome Segregation
Erythroid Cells
Mutation
Cytoskeletal Proteins
Cytokinesis
Eukaryotic Cells
Protein Binding
Genes
Cell Movement
Organism Cloning
Embryonic Structures
Chromosomes
Apoptosis

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Paw, B. H., Davidson, A. J., Zhou, Y., Li, R., Pratt, S. J., Lee, C., ... Zon, L. I. (2003). Cell-specific mitotic defect and dyserythropoiesis associated with erythroid band 3 deficiency. Nature Genetics, 34(1), 59-64. https://doi.org/10.1038/ng1137

Cell-specific mitotic defect and dyserythropoiesis associated with erythroid band 3 deficiency. / Paw, Barry H.; Davidson, Alan J.; Zhou, Yi; Li, Rong; Pratt, Stephen J.; Lee, Charles; Trede, Nikolaus S.; Brownlie, Alison; Donovan, Adriana; Liao, Eric C.; Ziai, James M.; Drejer, Anna H.; Guo, Wen; Kim, Carol H.; Gwynn, Babette; Peters, Luanne L.; Chernova, Marina N.; Alper, Seth L.; Zapata, Agustin; Wickramasinghe, Sunitha N.; Lee, Matthew J.; Lux, Samuel E.; Fritz, Andreas; Postlethwait, John H.; Zon, Leonard I.

In: Nature Genetics, Vol. 34, No. 1, 01.05.2003, p. 59-64.

Research output: Contribution to journalArticle

Paw, BH, Davidson, AJ, Zhou, Y, Li, R, Pratt, SJ, Lee, C, Trede, NS, Brownlie, A, Donovan, A, Liao, EC, Ziai, JM, Drejer, AH, Guo, W, Kim, CH, Gwynn, B, Peters, LL, Chernova, MN, Alper, SL, Zapata, A, Wickramasinghe, SN, Lee, MJ, Lux, SE, Fritz, A, Postlethwait, JH & Zon, LI 2003, 'Cell-specific mitotic defect and dyserythropoiesis associated with erythroid band 3 deficiency', Nature Genetics, vol. 34, no. 1, pp. 59-64. https://doi.org/10.1038/ng1137
Paw, Barry H. ; Davidson, Alan J. ; Zhou, Yi ; Li, Rong ; Pratt, Stephen J. ; Lee, Charles ; Trede, Nikolaus S. ; Brownlie, Alison ; Donovan, Adriana ; Liao, Eric C. ; Ziai, James M. ; Drejer, Anna H. ; Guo, Wen ; Kim, Carol H. ; Gwynn, Babette ; Peters, Luanne L. ; Chernova, Marina N. ; Alper, Seth L. ; Zapata, Agustin ; Wickramasinghe, Sunitha N. ; Lee, Matthew J. ; Lux, Samuel E. ; Fritz, Andreas ; Postlethwait, John H. ; Zon, Leonard I. / Cell-specific mitotic defect and dyserythropoiesis associated with erythroid band 3 deficiency. In: Nature Genetics. 2003 ; Vol. 34, No. 1. pp. 59-64.
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