Cell receptor specific targeted toxins: Genetic construction and characterization of an interleukin 2 diphtheria toxin-related fusion protein

J. R. Murphy, D. P. Williams, P. Bacha, W. Bishai, C. Waters, T. B. Strom

Research output: Contribution to journalArticle

Abstract

We have genetically replaced the diphtheria toxin receptor binding domain with a DNA insert encoding the T-cell growth factor interleukin-2 (IL-2). The toxin-related IL-2 fusion gene encodes a 70,586 dalton protein, pro-IL-2-toxin. The mature form of IL-2-toxin is exported to the periplasmic space of recombinant Escherichia coli and has a molecular weight of 68,086. IL-2-toxin has been partially purified from periplasmic extracts of recombinant E. coli, and it is shown to contain immunologic determinants intrinsic to both its diphtheria toxin and IL-2 components. The chimeric toxin is targeted toward IL-2 receptor bearing T-cells in vitro.

Original languageEnglish (US)
Pages (from-to)467-480
Number of pages14
JournalJournal of Receptors and Signal Transduction
Volume8
Issue number1-4
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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