Cell proliferation after ischemic infarction in gerbil brain

Monica Du Bois, Phillip D. Bowman, Gary W. Goldstein

Research output: Contribution to journalArticlepeer-review

Abstract

In order to study cell proliferation after ischemic infarction, a model of bilateral common carotid artery occlusion in the gerbil was developed. A comparison of survival rates after 15, 30, 45 and 60 min of occlusion revealed that 45 min was the maximum duration of ischemia after which most (72%) of the gerbils were alive at 1 week. The administration of pentobarbital (single dose, 30 mg/kg) post-operatively to badly seizing animals increased survival to 100%. Large, well-demarcated infarcts were present in posterior thalamus or midbrain in 62% of gerbils subjected to 45 min bilateral occlusion. In 60% of these animals the infarcts were unilateral; in 40% they were bilateral. To quantitate cell proliferation in the infarcts from 12 h to 25 days after ischemia, gerbils were injected with [3H]thymidine 4 h prior to sacrifice, and autoradiographs were prepared from sectioned brains. Proliferation took place from 2 to 7 days after occlusion, with a maximum of 24% labeled cells at 6 days.

Original languageEnglish (US)
Pages (from-to)245-252
Number of pages8
JournalBrain research
Volume347
Issue number2
DOIs
StatePublished - Nov 18 1985
Externally publishedYes

Keywords

  • [H]thymidine incorporation
  • gerbil
  • infarction
  • ischemia
  • macrophage

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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