Cell motility of neural stem cells is reduced after SPIO-labeling, which is mitigated after exocytosis

Stacey M. Cromer Berman; Kshitiz; C. Joanne Wang; Inema Orukari; Andre Levchenko; Jeff W.M. Bulte; Piotr Walczak

doi:10.1002/mrm.24216

Cell motility of neural stem cells is reduced after SPIO-labeling, which is mitigated after exocytosis

Stacey M. Cromer Berman, Kshitiz, C. Joanne Wang, Inema Orukari, Andre Levchenko, Jeff W.M. Bulte, Piotr Walczak

School of Medicine

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

MRI is used for tracking of superparamagnetic iron oxide (SPIO)-labeled neural stem cells. Studies have shown that long-term MR tracking of rapidly dividing cells underestimates their migration distance. Time-lapse microscopy of random cellular motility and cell division was performed to evaluate the effects of SPIO-labeling on neural stem cell migration. Labeled cells divided symmetrically and exhibited no changes in cell viability, proliferation, or apoptosis. However, SPIO-labeling resulted in decreased motility of neural stem cells as compared with unlabeled controls. When SPIO-labeled neural stem cells and human induced pluripotent stem cells were transplanted into mouse brain, rapid exocytosis of SPIO by live cells was observed as early as 48 h postengraftment, with SPIO-depleted cells showing the farthest migration distance. As label dilution is negligible at this early time point, we conclude that MRI underestimation of cell migration can also occur as a result of reduced cell motility, which appears to be mitigated following SPIO exocytosis. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.

Original language	English (US)
Pages (from-to)	255-262
Number of pages	8
Journal	Magnetic resonance in medicine
Volume	69
Issue number	1
DOIs	https://doi.org/10.1002/mrm.24216
State	Published - Jan 2013

Keywords

cell tracking
exocytosis
neural stem cell
superparamagnetic iron oxide

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

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10.1002/mrm.24216

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title = "Cell motility of neural stem cells is reduced after SPIO-labeling, which is mitigated after exocytosis",

abstract = "MRI is used for tracking of superparamagnetic iron oxide (SPIO)-labeled neural stem cells. Studies have shown that long-term MR tracking of rapidly dividing cells underestimates their migration distance. Time-lapse microscopy of random cellular motility and cell division was performed to evaluate the effects of SPIO-labeling on neural stem cell migration. Labeled cells divided symmetrically and exhibited no changes in cell viability, proliferation, or apoptosis. However, SPIO-labeling resulted in decreased motility of neural stem cells as compared with unlabeled controls. When SPIO-labeled neural stem cells and human induced pluripotent stem cells were transplanted into mouse brain, rapid exocytosis of SPIO by live cells was observed as early as 48 h postengraftment, with SPIO-depleted cells showing the farthest migration distance. As label dilution is negligible at this early time point, we conclude that MRI underestimation of cell migration can also occur as a result of reduced cell motility, which appears to be mitigated following SPIO exocytosis. Magn Reson Med, 2013. {\textcopyright} 2012 Wiley Periodicals, Inc.",

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AU - Cromer Berman, Stacey M.

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AU - Orukari, Inema

AU - Levchenko, Andre

AU - Bulte, Jeff W.M.

AU - Walczak, Piotr

PY - 2013/1

Y1 - 2013/1

N2 - MRI is used for tracking of superparamagnetic iron oxide (SPIO)-labeled neural stem cells. Studies have shown that long-term MR tracking of rapidly dividing cells underestimates their migration distance. Time-lapse microscopy of random cellular motility and cell division was performed to evaluate the effects of SPIO-labeling on neural stem cell migration. Labeled cells divided symmetrically and exhibited no changes in cell viability, proliferation, or apoptosis. However, SPIO-labeling resulted in decreased motility of neural stem cells as compared with unlabeled controls. When SPIO-labeled neural stem cells and human induced pluripotent stem cells were transplanted into mouse brain, rapid exocytosis of SPIO by live cells was observed as early as 48 h postengraftment, with SPIO-depleted cells showing the farthest migration distance. As label dilution is negligible at this early time point, we conclude that MRI underestimation of cell migration can also occur as a result of reduced cell motility, which appears to be mitigated following SPIO exocytosis. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.

AB - MRI is used for tracking of superparamagnetic iron oxide (SPIO)-labeled neural stem cells. Studies have shown that long-term MR tracking of rapidly dividing cells underestimates their migration distance. Time-lapse microscopy of random cellular motility and cell division was performed to evaluate the effects of SPIO-labeling on neural stem cell migration. Labeled cells divided symmetrically and exhibited no changes in cell viability, proliferation, or apoptosis. However, SPIO-labeling resulted in decreased motility of neural stem cells as compared with unlabeled controls. When SPIO-labeled neural stem cells and human induced pluripotent stem cells were transplanted into mouse brain, rapid exocytosis of SPIO by live cells was observed as early as 48 h postengraftment, with SPIO-depleted cells showing the farthest migration distance. As label dilution is negligible at this early time point, we conclude that MRI underestimation of cell migration can also occur as a result of reduced cell motility, which appears to be mitigated following SPIO exocytosis. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.

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Cell motility of neural stem cells is reduced after SPIO-labeling, which is mitigated after exocytosis

Abstract

Keywords

ASJC Scopus subject areas

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