TY - JOUR
T1 - Cell-mediated immune response is better preserved by laparoscopy than laparotomy
AU - Gitzelmann, Christopher A.
AU - Mendoza-Sagaon, Mario
AU - Talamini, Mark A.
AU - Ahmad, Syed A.
AU - Pegoli, Walter
AU - Paidas, Charles N.
N1 - Funding Information:
Supported in part by grants from Ethicon Endosurgery, Cincinnati, Ohio, and the Robert Garret Fund for the Surgical Treatment of Children, Baltimore, Md. C. A. Gitzelmann was supported by a grant from the Swiss National Research Foundation, Bern, Switzerland. M. Mendoza-Sagaon was supported in part by “Universidad Nacional Autonoma de Mexico.”
PY - 2000
Y1 - 2000
N2 - Background. This study compares the effects of carbon dioxide pneumoperitoneum versus laparotomy on cellular-mediated immune response in a murine model. Methods. Sixty-eight female C3H/He mice were sensitized to keyhole limpet hemocyanin (KLH) and to a mouse mammary carcinoma cell line (MC2) before surgery. Animals were randomized into 4 groups: group I, anesthesia (control); group II, pneumoperitoneum with carbon dioxide; group III, extraperitoneal wound; group IV, laparotomy. All animals were challenged subsequently with KLH and MC2 tumor cells. Delayed-type hypersensitivity skin reaction (DTH) to KLH was measured on postoperative days (PODs) 1, 2, 4, and 5. Tumor growth was assessed weekly as an indicator of postoperative cellular immune response. Results. Compared with preoperative values, postoperative DTH skin reactions were significantly less for all PODs in groups III and IV (P < .05), on POD 1 and 4 in group II (P < .05) and POD 4 for group I (P <. 05). Group IV showed significantly fewer DTH skin reactions for all PODs compared with groups I and II (P < .05) and all PODs except on day 2 compared with group III (P < . 05). Tumor growth was significantly increased at postoperative week 2 (n = 3/17 mice) and 3 (n = 4/17 mice) in group IV, when compared with groups I and II (P < . 05). Conclusions. Cellular immunity is preserved after carbon dioxide pneumoperitoneum compared with extraperitoneal incisions and laparotomy as measured by DTH and the ability to reject an immunogenictumor.
AB - Background. This study compares the effects of carbon dioxide pneumoperitoneum versus laparotomy on cellular-mediated immune response in a murine model. Methods. Sixty-eight female C3H/He mice were sensitized to keyhole limpet hemocyanin (KLH) and to a mouse mammary carcinoma cell line (MC2) before surgery. Animals were randomized into 4 groups: group I, anesthesia (control); group II, pneumoperitoneum with carbon dioxide; group III, extraperitoneal wound; group IV, laparotomy. All animals were challenged subsequently with KLH and MC2 tumor cells. Delayed-type hypersensitivity skin reaction (DTH) to KLH was measured on postoperative days (PODs) 1, 2, 4, and 5. Tumor growth was assessed weekly as an indicator of postoperative cellular immune response. Results. Compared with preoperative values, postoperative DTH skin reactions were significantly less for all PODs in groups III and IV (P < .05), on POD 1 and 4 in group II (P < .05) and POD 4 for group I (P <. 05). Group IV showed significantly fewer DTH skin reactions for all PODs compared with groups I and II (P < .05) and all PODs except on day 2 compared with group III (P < . 05). Tumor growth was significantly increased at postoperative week 2 (n = 3/17 mice) and 3 (n = 4/17 mice) in group IV, when compared with groups I and II (P < . 05). Conclusions. Cellular immunity is preserved after carbon dioxide pneumoperitoneum compared with extraperitoneal incisions and laparotomy as measured by DTH and the ability to reject an immunogenictumor.
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U2 - 10.1067/msy.2000.101152
DO - 10.1067/msy.2000.101152
M3 - Article
C2 - 10660760
AN - SCOPUS:0033978024
VL - 127
SP - 65
EP - 71
JO - Surgery
JF - Surgery
SN - 0039-6060
IS - 1
ER -