Cell fusion during yeast mating requires high levels of a-factor mating pheromone

Valeria Brizzio, Alison E. Gammie, Gaby Nijbroek, Susan Doris Michaelis, Mark D. Rose

Research output: Contribution to journalArticle

Abstract

During conjugation, two yeast cells fuse to form a single zygote. Cell fusion requires extensive re modeling of the cell wall, both to form a seal between the two cells and to remove the intervening material. The two plasma membranes then fuse to produce a continuous cytoplasm. We report the characterization of two cell fusion defective (Fus-) mutants, fus5 and fus8, isolated previously in our laboratory. Fluorescence and electron microscopy demonstrated that the fus5 and fus8 mutant zygotes were defective for cell wall remodeling/removal but not plasma membrane fusion. Strikingly, fus5 and fus8 were a specific; both mutations caused the mutant phenotype when present in the MATa parent but not in the MATα parent. Consistent with an a-specific defect, the fus5 and fus8 mutants produced less a-factor than the isogenic wild-type strain. FUS5 and FUS8 were determined to be allelic to AXL1 and RAM1, respectively, two genes known to be required for biogenesis of a-factor. Several experiments demonstrated that the partial defect in a-factor production resulted in the Fus- phenotype. First, overexpression of a-factor in the fus mutants suppressed the Fus defect. Second, matings to an MATα partner supersensitive to mating pheromone (sst2Δ) suppressed the Fus- defect in trans. Finally, the gent encoding a- factor, MFA1, was placed under the control of a repressible promoter; reduced levels of wild-type a-factor caused an identical cell fusion defect during mating. We conclude that high levels of pheromone are required as one component of the signal for prezygotes to initiate cell fusion.

Original languageEnglish (US)
Pages (from-to)1727-1739
Number of pages13
JournalJournal of Cell Biology
Volume135
Issue number6 II
DOIs
StatePublished - 1996

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Mating Factor
Cell Fusion
Yeasts
Zygote
Pheromones
Cell Wall
Cell Membrane
Phenotype
Membrane Fusion
Fluorescence Microscopy
Electron Microscopy
Cytoplasm
Mutation
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cell fusion during yeast mating requires high levels of a-factor mating pheromone. / Brizzio, Valeria; Gammie, Alison E.; Nijbroek, Gaby; Michaelis, Susan Doris; Rose, Mark D.

In: Journal of Cell Biology, Vol. 135, No. 6 II, 1996, p. 1727-1739.

Research output: Contribution to journalArticle

Brizzio, Valeria ; Gammie, Alison E. ; Nijbroek, Gaby ; Michaelis, Susan Doris ; Rose, Mark D. / Cell fusion during yeast mating requires high levels of a-factor mating pheromone. In: Journal of Cell Biology. 1996 ; Vol. 135, No. 6 II. pp. 1727-1739.
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