Cell fusion assays for yeast mating pairs

Eric Grote

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.

Original languageEnglish (US)
Title of host publicationCell Fusion
Subtitle of host publicationOverviews and Methods
PublisherHumana Press
Pages165-196
Number of pages32
ISBN (Print)9781588299116
DOIs
StatePublished - Jan 1 2008

Publication series

NameMethods in Molecular Biology
Volume475
ISSN (Print)1064-3745

Keywords

  • Cell fusion
  • Cell wall remodeling
  • Karyogamy
  • Mating
  • Membrane fusion

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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