Cell fusion assays for yeast mating pairs.

Eric Grote

Research output: Contribution to journalArticle

Abstract

Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.

Original languageEnglish (US)
Pages (from-to)165-196
Number of pages32
JournalMethods in molecular biology (Clifton, N.J.)
Volume475
StatePublished - 2008

Fingerprint

Cell Fusion
Yeasts
Nuclear Fusion
Membrane Fusion
Pheromones
Eukaryotic Cells
Cell Wall
Cell Membrane
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Cell fusion assays for yeast mating pairs. / Grote, Eric.

In: Methods in molecular biology (Clifton, N.J.), Vol. 475, 2008, p. 165-196.

Research output: Contribution to journalArticle

@article{6bd9d9f229034f4b8201c74d1b606cc4,
title = "Cell fusion assays for yeast mating pairs.",
abstract = "Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.",
author = "Eric Grote",
year = "2008",
language = "English (US)",
volume = "475",
pages = "165--196",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Cell fusion assays for yeast mating pairs.

AU - Grote, Eric

PY - 2008

Y1 - 2008

N2 - Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.

AB - Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.

UR - http://www.scopus.com/inward/record.url?scp=58149142567&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149142567&partnerID=8YFLogxK

M3 - Article

VL - 475

SP - 165

EP - 196

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -