Cell cycle-specific behavior of erythropoietin

Jerry L Spivak, D. K. Ferris, J. Fisher, S. J. Noga, M. Isaacs, E. Connor, W. D. Hankins

Research output: Contribution to journalArticle

Abstract

The murine erythropoietin-dependent erythroleukemia cell line, HCD-57, was employed to study the cell cycle-specific behavior of erythropoietin. Cell cycle duration for HCD-57 cells was approximately 12 hours and was uninfluenced by erythropoietin. Populations of HCD-57 cells synchronized in G1 by centrifugal elutriation were able to pass through one complete cell cycle in the absence of erythropoietin but, thereafter, arrested in G1 as identified by propidium iodide staining and flow cytometry. Analysis of cell cycle behavior using the metachromic dye acridine orange, however, revealed that HCD-57 cells pass through a G0 cell cycle phase and, like serum-deprived 3T3 cells, actually arrest in G0 when deprived of erythropoietin. Expression of the cell cycle regulatory protein p34cdc2 was invariant throughout the cell cycle in HCD-57 cells. p34cdc2 was constitutively phosphorylated in G0 cells, and this effect was not modified by erythropoietin. Erythropoietin receptor distribution was log normal in HCD-57 cells in each phase of the cell cycle. The affinity of these surface receptors for erythropoietin was essentially invariant throughout the cell cycle, but receptor expression was upregulated in G2M cells as compared with cells in G1 or S phase. Taken together, these data indicate that erythropoietin has an important role in the G0-G1 to S phase transition but, based on receptor expression, is involved in other phases of the cell cycle as well.

Original languageEnglish (US)
Pages (from-to)141-150
Number of pages10
JournalExperimental Hematology
Volume24
Issue number2
StatePublished - 1996

Fingerprint

Erythropoietin
Cell Cycle
Erythropoietin Receptors
S Phase
3T3 Cells
Leukemia, Erythroblastic, Acute
Cell Cycle Proteins
Acridine Orange
Propidium
Phase Transition
G1 Phase
Flow Cytometry
Coloring Agents
Staining and Labeling
Cell Line
Serum

Keywords

  • cell cycle
  • erythropoietin
  • erythropoietin receptor
  • p34cdc2

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Spivak, J. L., Ferris, D. K., Fisher, J., Noga, S. J., Isaacs, M., Connor, E., & Hankins, W. D. (1996). Cell cycle-specific behavior of erythropoietin. Experimental Hematology, 24(2), 141-150.

Cell cycle-specific behavior of erythropoietin. / Spivak, Jerry L; Ferris, D. K.; Fisher, J.; Noga, S. J.; Isaacs, M.; Connor, E.; Hankins, W. D.

In: Experimental Hematology, Vol. 24, No. 2, 1996, p. 141-150.

Research output: Contribution to journalArticle

Spivak, JL, Ferris, DK, Fisher, J, Noga, SJ, Isaacs, M, Connor, E & Hankins, WD 1996, 'Cell cycle-specific behavior of erythropoietin', Experimental Hematology, vol. 24, no. 2, pp. 141-150.
Spivak JL, Ferris DK, Fisher J, Noga SJ, Isaacs M, Connor E et al. Cell cycle-specific behavior of erythropoietin. Experimental Hematology. 1996;24(2):141-150.
Spivak, Jerry L ; Ferris, D. K. ; Fisher, J. ; Noga, S. J. ; Isaacs, M. ; Connor, E. ; Hankins, W. D. / Cell cycle-specific behavior of erythropoietin. In: Experimental Hematology. 1996 ; Vol. 24, No. 2. pp. 141-150.
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