Cell cycle kinetic studies in 68 patients with myelodysplastic syndromes following intravenous iodo- and/or bromodeoxyuridine

A. Raza, S. Alvi, L. Broady-Robinson, M. Showel, J. Cartlidge, S. D. Mundle, V. T. Shetty, R. Z. Borok, S. E. Dar, H. K. Chopra, L. Span, A. Parcharidou, C. Hines, S. Gezer, P. Venugopal, J. Loew, J. Showel, D. Alston, B. Hernandez, S. RifkinE. Robin, R. Shah, S. A. Gregory

Research output: Contribution to journalArticle

Abstract

Sixty-eight patients with myelodysplastic syndromes (MDS) received sequential infusions of iodo- and/or bromodeoxyuridine for cell kinetic analysis. Bone marrow biopsy sections were treated by appropriate antibodies and a labeling index (LI), duration of S-phase (Ts), and total cell cycle time (Tc) of myeloid cells were determined. The mean LI was 28.4%, Ts was 11.8 hours and Tc was 40.7 hours. The %LI decreased as the disease evolved from refractory anemia toward transformation to acute leukemia (p = 0.04). Double-labeling of biopsy sections for apoptosis and proliferation showed that 30-90% of S-phase cells in MDS patients were simultaneously apoptotic or 'antonymous.' We conclude that MDS are highly proliferative disorders in which the ineffective hematopoiesis is probably the result of excessive apoptosis rather than slow proliferation.

Original languageEnglish (US)
Pages (from-to)530-535
Number of pages6
JournalExperimental Hematology
Volume25
Issue number6
StatePublished - Jul 24 1997

Keywords

  • Antonymy
  • Apoptosis
  • Myelodysplastic Syndromes (MDS)
  • Proliferation

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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    Raza, A., Alvi, S., Broady-Robinson, L., Showel, M., Cartlidge, J., Mundle, S. D., Shetty, V. T., Borok, R. Z., Dar, S. E., Chopra, H. K., Span, L., Parcharidou, A., Hines, C., Gezer, S., Venugopal, P., Loew, J., Showel, J., Alston, D., Hernandez, B., ... Gregory, S. A. (1997). Cell cycle kinetic studies in 68 patients with myelodysplastic syndromes following intravenous iodo- and/or bromodeoxyuridine. Experimental Hematology, 25(6), 530-535.