TY - JOUR
T1 - Cell cycle analysis using bromodeoxyuridine
T2 - Comparison of methods for analysis of total cell transit time
AU - Trent, Jeffrey M.
AU - Gerner, Eugene
AU - Broderick, Rebecca
AU - Crossen, Peter E.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - Results are presented supporting the study of cellular proliferation utilizing 5-bromodeoxyuridine (BrdU) incorporation followed by sister chromatid differential staining. In order to determine the relative accuracy of this method in estimating total cell transit time (Tc), we utilized a thermosensitive rat embryonic cell line to compare measurement of Tc based on the percent differentially labeled (PDLM) technique, with cell cycle measurements using [3H]-thymidine ([3H]-TdR) incorporation and the percent labeled mitoses (PLM) technique. Results of PLM and PDLM analysis were shown to be highly concordant, indicating the utility of the BrdU method for analysis of Tc. Results are presented suggesting the general application of the PDLM method for estimations of Tc in cultures of human tumors.
AB - Results are presented supporting the study of cellular proliferation utilizing 5-bromodeoxyuridine (BrdU) incorporation followed by sister chromatid differential staining. In order to determine the relative accuracy of this method in estimating total cell transit time (Tc), we utilized a thermosensitive rat embryonic cell line to compare measurement of Tc based on the percent differentially labeled (PDLM) technique, with cell cycle measurements using [3H]-thymidine ([3H]-TdR) incorporation and the percent labeled mitoses (PLM) technique. Results of PLM and PDLM analysis were shown to be highly concordant, indicating the utility of the BrdU method for analysis of Tc. Results are presented suggesting the general application of the PDLM method for estimations of Tc in cultures of human tumors.
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U2 - 10.1016/0165-4608(86)90370-5
DO - 10.1016/0165-4608(86)90370-5
M3 - Article
C2 - 2415235
AN - SCOPUS:0022611511
VL - 19
SP - 43
EP - 50
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
SN - 0165-4608
IS - 1-2
ER -