TY - JOUR
T1 - Cell cultures as models of cardiac mechanoelectric feedback
AU - Zhang, Yibing
AU - Sekar, Rajesh B.
AU - McCulloch, Andrew D.
AU - Tung, Leslie
N1 - Funding Information:
Funding for this work was provided by an AHA Mid-Atlantic Affiliate postdoctoral fellowship (to Y. Z.), National Institutes of Health Grants R01 HL66239 (to L.T.), R21 RR017073 (to L.T.), P01 HL46345 (to A.D.M.) and NSF Grant BES-0506252 (to A.D.M.).
PY - 2008/6
Y1 - 2008/6
N2 - Although stretch-activated currents have been extensively studied in isolated cells and intact heart in the context of mechanoelectric feedback (MEF) in the heart, quantitative data regarding other mechanical parameters such as pressure, shear, bending, etc, are still lacking at the multicellular level. Cultured cardiac cell monolayers have been used increasingly in the past decade as an in vitro model for the studies of fundamental mechanisms that underlie normal and pathological electrophysiology at the tissue level. Optical mapping makes possible multisite recording and analysis of action potentials and wavefront propagation, suitable for monitoring the electrophysiological activity of the cardiac cell monolayer under a wide variety of controlled mechanical conditions. In this paper, we review methodologies that have been developed or could be used to mechanically perturb cell monolayers, and present some new results on the acute effects of pressure, shear stress and anisotropic strain on cultured neonatal rat ventricular myocyte (NRVM) monolayers.
AB - Although stretch-activated currents have been extensively studied in isolated cells and intact heart in the context of mechanoelectric feedback (MEF) in the heart, quantitative data regarding other mechanical parameters such as pressure, shear, bending, etc, are still lacking at the multicellular level. Cultured cardiac cell monolayers have been used increasingly in the past decade as an in vitro model for the studies of fundamental mechanisms that underlie normal and pathological electrophysiology at the tissue level. Optical mapping makes possible multisite recording and analysis of action potentials and wavefront propagation, suitable for monitoring the electrophysiological activity of the cardiac cell monolayer under a wide variety of controlled mechanical conditions. In this paper, we review methodologies that have been developed or could be used to mechanically perturb cell monolayers, and present some new results on the acute effects of pressure, shear stress and anisotropic strain on cultured neonatal rat ventricular myocyte (NRVM) monolayers.
KW - Anisotropic stretch
KW - Cardiac cell monolayer
KW - Electrophysiology
KW - Mechanoelectrical feedback
KW - Optical mapping
KW - Pressure
KW - Shear stress
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U2 - 10.1016/j.pbiomolbio.2008.02.017
DO - 10.1016/j.pbiomolbio.2008.02.017
M3 - Review article
C2 - 18384846
AN - SCOPUS:46549086503
SN - 0079-6107
VL - 97
SP - 367
EP - 382
JO - Progress in Biophysics and Molecular Biology
JF - Progress in Biophysics and Molecular Biology
IS - 2-3
ER -