Cell calcium signaling mediates capillary regulation of blood flow in the Retina

Kyle R. Biesecker, Anja I. Srienc, Angela M. Shimoda, Amit Agarwal, Dwight E. Bergles, Paulo Kofuji, Eric A. Newman

Research output: Research - peer-reviewArticle

Abstract

The brain is critically dependent on the regulation of blood flow to nourish active neurons. One widely held hypothesis of blood flow regulation holds that active neurons stimulate Ca2+ increases in glial cells, triggering glial release of vasodilating agents. This hypothesis has been challenged, as arteriole dilation can occur in the absence of glial Ca2+ signaling. We address this controversy by imaging glial Ca2+ signaling and vessel dilation in the mouse retina. We find that sensory stimulation results in Ca2+ increases in the glial endfeet contacting capillaries, but not arterioles, and that capillary dilations often follow spontaneous Ca2+ signaling. In IP3R2−/− mice, where glial Ca2+ signaling is reduced, light-evoked capillary, but not arteriole, dilation is abolished. The results show that, independent of arterioles, capillaries actively dilate and regulate blood flow. Furthermore, the results demonstrate that glial Ca2+ signaling regulates capillary but not arteriole blood flow.

LanguageEnglish (US)
Pages9435-9445
Number of pages11
JournalJournal of Neuroscience
Volume36
Issue number36
DOIs
StatePublished - Sep 7 2016

Fingerprint

Calcium Signaling
Neuroglia
Retina
Arterioles
Dilatation
Neurons
Light
Brain

Keywords

  • Blood flow
  • Calcium signaling
  • Capillary
  • Glia
  • Neurovascular coupling
  • Retina

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Biesecker, K. R., Srienc, A. I., Shimoda, A. M., Agarwal, A., Bergles, D. E., Kofuji, P., & Newman, E. A. (2016). Cell calcium signaling mediates capillary regulation of blood flow in the Retina. Journal of Neuroscience, 36(36), 9435-9445. DOI: 10.1523/JNEUROSCI.1782-16.2016

Cell calcium signaling mediates capillary regulation of blood flow in the Retina. / Biesecker, Kyle R.; Srienc, Anja I.; Shimoda, Angela M.; Agarwal, Amit; Bergles, Dwight E.; Kofuji, Paulo; Newman, Eric A.

In: Journal of Neuroscience, Vol. 36, No. 36, 07.09.2016, p. 9435-9445.

Research output: Research - peer-reviewArticle

Biesecker, KR, Srienc, AI, Shimoda, AM, Agarwal, A, Bergles, DE, Kofuji, P & Newman, EA 2016, 'Cell calcium signaling mediates capillary regulation of blood flow in the Retina' Journal of Neuroscience, vol 36, no. 36, pp. 9435-9445. DOI: 10.1523/JNEUROSCI.1782-16.2016
Biesecker KR, Srienc AI, Shimoda AM, Agarwal A, Bergles DE, Kofuji P et al. Cell calcium signaling mediates capillary regulation of blood flow in the Retina. Journal of Neuroscience. 2016 Sep 7;36(36):9435-9445. Available from, DOI: 10.1523/JNEUROSCI.1782-16.2016
Biesecker, Kyle R. ; Srienc, Anja I. ; Shimoda, Angela M. ; Agarwal, Amit ; Bergles, Dwight E. ; Kofuji, Paulo ; Newman, Eric A./ Cell calcium signaling mediates capillary regulation of blood flow in the Retina. In: Journal of Neuroscience. 2016 ; Vol. 36, No. 36. pp. 9435-9445
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