Cell and tissue microdissection in combination with genomic and proteomic applications

Stephen D. Ginsberg, Scott E. Hemby, Elliott J. Mufson, Lee J. Martin

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The combination of tissue microdissection protocols including discrete cell microaspiration and laser capture microdissection with high throughput gene expression profiling platforms such as cDNA microarrays and oligonucleotide microarrays enables the simultaneous assessment of many individual elements from a single cell or a population of homogeneous cells. This chapter outlines in detail the theoretical and practical background for selecting the appropriate tissues and conditions amenable to expression profiling. In addition, this report illustrates the usage of microdissection strategies and RNA amplification methodologies in concert with array technologies using tissues harvested from the central nervous system obtained from animal models of neurodegeneration and postmortem human brain tissues.

Original languageEnglish (US)
Title of host publicationNeuroanatomical Tract-Tracing 3
Subtitle of host publicationMolecules, Neurons, and Systems
PublisherSpringer US
Pages109-141
Number of pages33
ISBN (Electronic)9780387289427
ISBN (Print)0387289410, 9780387289410
DOIs
StatePublished - Jan 1 2006

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Keywords

  • RNA amplification
  • SELDI-TOF
  • brain
  • expression profiling
  • laser capture microdissection
  • microarray
  • molecular fingerprint

ASJC Scopus subject areas

  • Medicine(all)
  • Neuroscience(all)

Cite this

Ginsberg, S. D., Hemby, S. E., Mufson, E. J., & Martin, L. J. (2006). Cell and tissue microdissection in combination with genomic and proteomic applications. In Neuroanatomical Tract-Tracing 3: Molecules, Neurons, and Systems (pp. 109-141). Springer US. https://doi.org/10.1007/0-387-28942-9_4