Abstract
The combination of tissue microdissection protocols including discrete cell microaspiration and laser capture microdissection with high throughput gene expression profiling platforms such as cDNA microarrays and oligonucleotide microarrays enables the simultaneous assessment of many individual elements from a single cell or a population of homogeneous cells. This chapter outlines in detail the theoretical and practical background for selecting the appropriate tissues and conditions amenable to expression profiling. In addition, this report illustrates the usage of microdissection strategies and RNA amplification methodologies in concert with array technologies using tissues harvested from the central nervous system obtained from animal models of neurodegeneration and postmortem human brain tissues.
Original language | English (US) |
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Title of host publication | Neuroanatomical Tract-Tracing 3 |
Subtitle of host publication | Molecules, Neurons, and Systems |
Publisher | Springer US |
Pages | 109-141 |
Number of pages | 33 |
ISBN (Electronic) | 9780387289427 |
ISBN (Print) | 0387289410, 9780387289410 |
DOIs | |
State | Published - Jan 1 2006 |
Keywords
- RNA amplification
- SELDI-TOF
- brain
- expression profiling
- laser capture microdissection
- microarray
- molecular fingerprint
ASJC Scopus subject areas
- Medicine(all)
- Neuroscience(all)