Cell and tissue microdissection in combination with genomic and proteomic applications

Stephen D. Ginsberg; Scott E. Hemby; Elliott J. Mufson; Lee J. Martin

doi:10.1007/0-387-28942-9_4

Cell and tissue microdissection in combination with genomic and proteomic applications

Stephen D. Ginsberg, Scott E. Hemby, Elliott J. Mufson, Lee J. Martin

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The combination of tissue microdissection protocols including discrete cell microaspiration and laser capture microdissection with high throughput gene expression profiling platforms such as cDNA microarrays and oligonucleotide microarrays enables the simultaneous assessment of many individual elements from a single cell or a population of homogeneous cells. This chapter outlines in detail the theoretical and practical background for selecting the appropriate tissues and conditions amenable to expression profiling. In addition, this report illustrates the usage of microdissection strategies and RNA amplification methodologies in concert with array technologies using tissues harvested from the central nervous system obtained from animal models of neurodegeneration and postmortem human brain tissues.

Original language	English (US)
Title of host publication	Neuroanatomical Tract-Tracing 3
Subtitle of host publication	Molecules, Neurons, and Systems
Publisher	Springer US
Pages	109-141
Number of pages	33
ISBN (Electronic)	9780387289427
ISBN (Print)	0387289410, 9780387289410
DOIs	https://doi.org/10.1007/0-387-28942-9_4
State	Published - Jan 1 2006

Keywords

RNA amplification
SELDI-TOF
brain
expression profiling
laser capture microdissection
microarray
molecular fingerprint

ASJC Scopus subject areas

Medicine(all)
Neuroscience(all)

Access to Document

10.1007/0-387-28942-9_4

Cite this

@inbook{33dc49c0d02a46ab9e1a8720b4e1daeb,

title = "Cell and tissue microdissection in combination with genomic and proteomic applications",

abstract = "The combination of tissue microdissection protocols including discrete cell microaspiration and laser capture microdissection with high throughput gene expression profiling platforms such as cDNA microarrays and oligonucleotide microarrays enables the simultaneous assessment of many individual elements from a single cell or a population of homogeneous cells. This chapter outlines in detail the theoretical and practical background for selecting the appropriate tissues and conditions amenable to expression profiling. In addition, this report illustrates the usage of microdissection strategies and RNA amplification methodologies in concert with array technologies using tissues harvested from the central nervous system obtained from animal models of neurodegeneration and postmortem human brain tissues.",

keywords = "RNA amplification, SELDI-TOF, brain, expression profiling, laser capture microdissection, microarray, molecular fingerprint",

author = "Ginsberg, {Stephen D.} and Hemby, {Scott E.} and Mufson, {Elliott J.} and Martin, {Lee J.}",

year = "2006",

month = jan,

day = "1",

doi = "10.1007/0-387-28942-9_4",

language = "English (US)",

isbn = "0387289410",

pages = "109--141",

booktitle = "Neuroanatomical Tract-Tracing 3",

publisher = "Springer US",

}

TY - CHAP

T1 - Cell and tissue microdissection in combination with genomic and proteomic applications

AU - Ginsberg, Stephen D.

AU - Hemby, Scott E.

AU - Mufson, Elliott J.

AU - Martin, Lee J.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - The combination of tissue microdissection protocols including discrete cell microaspiration and laser capture microdissection with high throughput gene expression profiling platforms such as cDNA microarrays and oligonucleotide microarrays enables the simultaneous assessment of many individual elements from a single cell or a population of homogeneous cells. This chapter outlines in detail the theoretical and practical background for selecting the appropriate tissues and conditions amenable to expression profiling. In addition, this report illustrates the usage of microdissection strategies and RNA amplification methodologies in concert with array technologies using tissues harvested from the central nervous system obtained from animal models of neurodegeneration and postmortem human brain tissues.

AB - The combination of tissue microdissection protocols including discrete cell microaspiration and laser capture microdissection with high throughput gene expression profiling platforms such as cDNA microarrays and oligonucleotide microarrays enables the simultaneous assessment of many individual elements from a single cell or a population of homogeneous cells. This chapter outlines in detail the theoretical and practical background for selecting the appropriate tissues and conditions amenable to expression profiling. In addition, this report illustrates the usage of microdissection strategies and RNA amplification methodologies in concert with array technologies using tissues harvested from the central nervous system obtained from animal models of neurodegeneration and postmortem human brain tissues.

KW - RNA amplification

KW - SELDI-TOF

KW - brain

KW - expression profiling

KW - laser capture microdissection

KW - microarray

KW - molecular fingerprint

UR - http://www.scopus.com/inward/record.url?scp=77955397778&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955397778&partnerID=8YFLogxK

U2 - 10.1007/0-387-28942-9_4

DO - 10.1007/0-387-28942-9_4

M3 - Chapter

AN - SCOPUS:77955397778

SN - 0387289410

SN - 9780387289410

SP - 109

EP - 141

BT - Neuroanatomical Tract-Tracing 3

PB - Springer US

ER -

Cell and tissue microdissection in combination with genomic and proteomic applications

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this