Cell activation of human macrophages by lipoteichoic acid is strongly attenuated by lipopolysaccharide-binding protein

Mareike Mueller, Cordula Stamme, Christian Draing, Thomas Hartung, Ulrich Seydel, Andra B. Schromm

Research output: Contribution to journalArticle

Abstract

Lipoteichoic acid (LTA) represents immunostimulatory molecules expressed by Gram-positive bacteria. They activate the innate immune system via Toll-like receptors. We have investigated the role of serum proteins in activation of human macrophages by LTA from Staphylococcus aureus and found it to be strongly attenuated by serum. In contrast, the same cells showed a sensitive response to LTA and a significantly enhanced production of tumor necrosis factor α under serum-free conditions. We show that LTA interacts with the serum protein lipopolysaccharide-binding protein (LBP) and inhibits the integration of LBP into phospholipid membranes, indicating the formation of complexes of LTA and soluble LBP. The addition of recombinant human LBP to serum-free medium inhibited the production of tumor necrosis factor α and interleukins 6 and 8 after stimulation of human macrophages with LTA in a dose-dependent manner. Using anti-LBP antibodies, this inhibitory effect could be attributed to soluble LBP, whereas LBP in its recently described transmembrane configuration did not modulate cell activation. Also, using primary alveolar macrophages from rats, we show a sensitive cytokine response to LTA under serum-free culture conditions that was strongly attenuated in the presence of serum. In summary, our data suggest that innate immune recognition of LTA is organ-specific with negative regulation by LBP in serum-containing compartments and sensitive recognition in serum-free compartments like the lung.

Original languageEnglish (US)
Pages (from-to)31448-31456
Number of pages9
JournalJournal of Biological Chemistry
Volume281
Issue number42
DOIs
StatePublished - Oct 20 2006
Externally publishedYes

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Macrophage Activation
Macrophages
Chemical activation
Serum
Blood Proteins
Tumor Necrosis Factor-alpha
lipoteichoic acid
lipopolysaccharide-binding protein
Immune system
Toll-Like Receptors
Serum-Free Culture Media
Alveolar Macrophages
Gram-Positive Bacteria
Interleukin-8
Protein Binding
Staphylococcus aureus
Rats
Immune System
Interleukin-6
Phospholipids

ASJC Scopus subject areas

  • Biochemistry

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Cell activation of human macrophages by lipoteichoic acid is strongly attenuated by lipopolysaccharide-binding protein. / Mueller, Mareike; Stamme, Cordula; Draing, Christian; Hartung, Thomas; Seydel, Ulrich; Schromm, Andra B.

In: Journal of Biological Chemistry, Vol. 281, No. 42, 20.10.2006, p. 31448-31456.

Research output: Contribution to journalArticle

Mueller, Mareike ; Stamme, Cordula ; Draing, Christian ; Hartung, Thomas ; Seydel, Ulrich ; Schromm, Andra B. / Cell activation of human macrophages by lipoteichoic acid is strongly attenuated by lipopolysaccharide-binding protein. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 42. pp. 31448-31456.
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