C/EBP-β mediates iNOS induction by hypoxia in rat pulmonary microvascular smooth muscle cells

Xingwu Teng, Dechun Li, John D. Catravas, Roger A. Johns

Research output: Contribution to journalArticle

Abstract

Exposure of rats to 10% O2 for 4 days caused pulmonary hypertension and induced expression of both inducible nitric oxide synthase (iNOS) and CCAAT box enhancer binding protein-β (C/EBP-β) in rat lung. Electrophoretic mobility shift assays (EMSAs) showed that exposure to 1% O2 increased the C/EBP-β binding in rat pulmonary microvascular smooth muscle cells (rPSMs). To test the hypothesis that C/EBP-β participates in hypoxia-induced iNOS expression in rPSMs, a C/EBP motif at -910 bp of rat iNOS promoter was mutated. rPSMs transfected with the rat iNOS promoter and exposed to 1% O2 for 24 hours had significantly increased wild-type iNOS promoter activity. The hypoxia-induced promoter activity was abolished by the C/EBP motif mutation. Thus, C/EBP-β mediates, at least in part, hypoxia-induced iNOS expression in rPSMs.

Original languageEnglish (US)
Pages (from-to)125-127
Number of pages3
JournalCirculation research
Volume90
Issue number2
DOIs
StatePublished - Feb 8 2002

Keywords

  • C/EBP-β
  • Gene regulation
  • Hypoxia
  • Inducible nitric oxide synthase
  • Pulmonary hypertension

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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