cDNA Cloning of Rabbit Thrombomodulin and Characterization of Gene Expression in Cardiovascular Tissue

Clayton B. Deming, Antony Y. Kim, C. E. Bian, Jean B. Regard, Jeffrey J. Rade

Research output: Contribution to journalArticlepeer-review

Abstract

Thrombomodulin (TM), a component of the protein C anticoagulant pathway, is critical for the maintenance of vascular thromboresistance. To facilitate the study of in vivo TM regulation, we cloned and sequenced the cDNA encoding full-length rabbit TM. Translation of the open reading frame predicts a 580 amino acid protein that contains a 19 amino acid signal peptide, one lectin-like and six EGF-like extracellular domains, a 23 amino acid transmembrane domain and a 36 amino acid cytoplasmic domain. In addition, there are three potential N-linked and six O-linked glycosylation sites. Comparison of the predicted rabbit TM protein with those of human, mouse and rat reveals 67-72% primary sequence conservation with identical domain homology. TM gene expression was quantified in rabbit cardiovascular tissue by real-time PCR using primers and probe based on the derived cDNA sequence and found to correlate with protein expression as determined by Western blot analysis.

Original languageEnglish (US)
Pages (from-to)399-405
Number of pages7
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume14
Issue number6
DOIs
StatePublished - Dec 1 2003

Keywords

  • DNA Sequence
  • Rabbit
  • Thrombomodulin
  • cDNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

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