cDNA cloning and characterization of vinculin mRNA

M. D. Coutu; D. J. Simon; A. E. Brown; S. W. Craig

doi:10.1016/0006-291X(87)90978-8

cDNA cloning and characterization of vinculin mRNA

M. D. Coutu, D. J. Simon, A. E. Brown, S. W. Craig

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Vinculin and meta-vinculin are related proteins that localize to adherens junctions. To facilitate studies on the biology of these proteins cDNA clones were isolated from a chick embryonic library made in λ gt 11. Three clones, accounting for 1 2 of the vinculin message, were identified by immunological analyses of fusion proteins and by hybrid-arrested translation. Sequence analyses of the 3′ ends of these clones reveal allelic variants. Northern blot analyses suggests the presence of multiple vinculin messages, the most prominent one at 6.2 kb, almost twice the size needed to encode vinculin.

Original language	English (US)
Pages (from-to)	637-643
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	147
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(87)90978-8
State	Published - Sep 15 1987

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(87)90978-8

Cite this

@article{cb600cac65fb409194820be12f356617,

title = "cDNA cloning and characterization of vinculin mRNA",

abstract = "Vinculin and meta-vinculin are related proteins that localize to adherens junctions. To facilitate studies on the biology of these proteins cDNA clones were isolated from a chick embryonic library made in λ gt 11. Three clones, accounting for 1 2 of the vinculin message, were identified by immunological analyses of fusion proteins and by hybrid-arrested translation. Sequence analyses of the 3′ ends of these clones reveal allelic variants. Northern blot analyses suggests the presence of multiple vinculin messages, the most prominent one at 6.2 kb, almost twice the size needed to encode vinculin.",

author = "Coutu, {M. D.} and Simon, {D. J.} and Brown, {A. E.} and Craig, {S. W.}",

year = "1987",

month = sep,

day = "15",

doi = "10.1016/0006-291X(87)90978-8",

language = "English (US)",

volume = "147",

pages = "637--643",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - cDNA cloning and characterization of vinculin mRNA

AU - Coutu, M. D.

AU - Simon, D. J.

AU - Brown, A. E.

AU - Craig, S. W.

PY - 1987/9/15

Y1 - 1987/9/15

N2 - Vinculin and meta-vinculin are related proteins that localize to adherens junctions. To facilitate studies on the biology of these proteins cDNA clones were isolated from a chick embryonic library made in λ gt 11. Three clones, accounting for 1 2 of the vinculin message, were identified by immunological analyses of fusion proteins and by hybrid-arrested translation. Sequence analyses of the 3′ ends of these clones reveal allelic variants. Northern blot analyses suggests the presence of multiple vinculin messages, the most prominent one at 6.2 kb, almost twice the size needed to encode vinculin.

AB - Vinculin and meta-vinculin are related proteins that localize to adherens junctions. To facilitate studies on the biology of these proteins cDNA clones were isolated from a chick embryonic library made in λ gt 11. Three clones, accounting for 1 2 of the vinculin message, were identified by immunological analyses of fusion proteins and by hybrid-arrested translation. Sequence analyses of the 3′ ends of these clones reveal allelic variants. Northern blot analyses suggests the presence of multiple vinculin messages, the most prominent one at 6.2 kb, almost twice the size needed to encode vinculin.

UR - http://www.scopus.com/inward/record.url?scp=0023500851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023500851&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(87)90978-8

DO - 10.1016/0006-291X(87)90978-8

M3 - Article

C2 - 2820410

AN - SCOPUS:0023500851

VL - 147

SP - 637

EP - 643

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

cDNA cloning and characterization of vinculin mRNA

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this