cDNA cloning and characterization of vinculin mRNA

M. D. Coutu; D. J. Simon; A. E. Brown; S. W. Craig

doi:10.1016/0006-291X(87)90978-8

cDNA cloning and characterization of vinculin mRNA

M. D. Coutu, D. J. Simon, A. E. Brown, S. W. Craig

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Vinculin and meta-vinculin are related proteins that localize to adherens junctions. To facilitate studies on the biology of these proteins cDNA clones were isolated from a chick embryonic library made in λ gt 11. Three clones, accounting for 1 2 of the vinculin message, were identified by immunological analyses of fusion proteins and by hybrid-arrested translation. Sequence analyses of the 3′ ends of these clones reveal allelic variants. Northern blot analyses suggests the presence of multiple vinculin messages, the most prominent one at 6.2 kb, almost twice the size needed to encode vinculin.

Original language	English (US)
Pages (from-to)	637-643
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	147
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(87)90978-8
State	Published - Sep 15 1987

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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