cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma

C. L. Martens, T. F. Huff, P. Jardieu, M. L. Trounstine, R. L. Coffman, K. Ishizaka, K. W. Moore

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Abstract

cDNA clones encoding rodent IgE-binding factors (IgE-BF) were isolated from cDNA libraries of a rat-mouse T hybridoma that secretes IgE-suppressive factor (IgE-SF) upon incubation with rat IgE. COS7 cells transfected with two of the cDNAs expressed IgE-BF, which selectively potentiate an in vitro IgE response. IgE-BF expressed in COS7 cells are glycoproteins of ~60 and ~11 kDa. DNA sequence analysis of an IgE-BF cDNA revealed a 556-amino acid (62 kDa) protein coding region. The results suggest that IgE-potentiating and IgE-suppressive factors share common precursor polypeptides and that the 11-kDa IgE-BF is derived from a 60-kDa precursor.

Original languageEnglish (US)
Pages (from-to)2460-2464
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number8
DOIs
StatePublished - Sep 11 1985

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Martens, C. L., Huff, T. F., Jardieu, P., Trounstine, M. L., Coffman, R. L., Ishizaka, K., & Moore, K. W. (1985). cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma. Proceedings of the National Academy of Sciences of the United States of America, 82(8), 2460-2464. https://doi.org/10.1073/pnas.82.8.2460