cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma

C. L. Martens, T. F. Huff, P. Jardieu, M. L. Trounstine, R. L. Coffman, K. Ishizaka, K. W. Moore

Research output: Contribution to journalArticle

Abstract

cDNA clones encoding rodent IgE-binding factors (IgE-BF) were isolated from cDNA libraries of a rat-mouse T hybridoma that secretes IgE-suppressive factor (IgE-SF) upon incubation with rat IgE. COS7 cells transfected with two of the cDNAs expressed IgE-BF, which selectively potentiate an in vitro IgE response. IgE-BF expressed in COS7 cells are glycoproteins of ~60 and ~11 kDa. DNA sequence analysis of an IgE-BF cDNA revealed a 556-amino acid (62 kDa) protein coding region. The results suggest that IgE-potentiating and IgE-suppressive factors share common precursor polypeptides and that the 11-kDa IgE-BF is derived from a 60-kDa precursor.

Original languageEnglish (US)
Pages (from-to)2460-2464
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number8
DOIs
StatePublished - 1985
Externally publishedYes

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Hybridomas
Complementary DNA
Clone Cells
T-Lymphocytes
Immunoglobulin E
Gene Library
DNA Sequence Analysis
Open Reading Frames
immunoglobulin-binding factors
Rodentia
Glycoproteins
Amino Acids
Peptides

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Martens, C. L., Huff, T. F., Jardieu, P., Trounstine, M. L., Coffman, R. L., Ishizaka, K., & Moore, K. W. (1985). cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma. Proceedings of the National Academy of Sciences of the United States of America, 82(8), 2460-2464. https://doi.org/10.1073/pnas.82.8.2460

cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma. / Martens, C. L.; Huff, T. F.; Jardieu, P.; Trounstine, M. L.; Coffman, R. L.; Ishizaka, K.; Moore, K. W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 8, 1985, p. 2460-2464.

Research output: Contribution to journalArticle

Martens, CL, Huff, TF, Jardieu, P, Trounstine, ML, Coffman, RL, Ishizaka, K & Moore, KW 1985, 'cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma', Proceedings of the National Academy of Sciences of the United States of America, vol. 82, no. 8, pp. 2460-2464. https://doi.org/10.1073/pnas.82.8.2460
Martens, C. L. ; Huff, T. F. ; Jardieu, P. ; Trounstine, M. L. ; Coffman, R. L. ; Ishizaka, K. ; Moore, K. W. / cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma. In: Proceedings of the National Academy of Sciences of the United States of America. 1985 ; Vol. 82, No. 8. pp. 2460-2464.
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AU - Huff, T. F.

AU - Jardieu, P.

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AU - Coffman, R. L.

AU - Ishizaka, K.

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