Cdc42 and Rac1 are necessary for autotaxin-induced tumor cell motility in A2058 melanoma cells

In Duk Jung; Jangsoon Lee; Seong Young Yun; Chang Gyo Park; Wahn Soo Choi; Hyang Woo Lee; Oksoon H. Choi; Jeung Whan Han; Hoi Young Lee

doi:10.1016/S0014-5793(02)03698-0

Cdc42 and Rac1 are necessary for autotaxin-induced tumor cell motility in A2058 melanoma cells

In Duk Jung, Jangsoon Lee, Seong Young Yun, Chang Gyo Park, Wahn Soo Choi, Hyang Woo Lee, Oksoon H. Choi, Jeung Whan Han, Hoi Young Lee

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Autotaxin (ATX) is a strong motogen that can increase invasiveness and angiogenesis. In the present study, we investigated the signal transduction mechanism of ATX-induced tumor cell motility. Unlike N19RhoA expressing cells, the cells expressing N17Cdc42 or N17Rac1 showed reduced motility against ATX. ATX activated Cdc42 and Rac1 and increased complex formation between these small G proteins and p21-activated kinase (PAK). Furthermore, ATX phosphorylated focal adhesion kinase (FAK) that was not shown in cells expressing dominant negative mutants of Cdc42 or Rac1. Collectively, these data strongly indicate that Cdc42 and Rac1 are essential for ATX-induced tumor cell motility in A2058 melanoma cells, and that PAK and FAK might be also involved in the process.

Original language	English (US)
Pages (from-to)	351-356
Number of pages	6
Journal	FEBS Letters
Volume	532
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(02)03698-0
State	Published - Dec 18 2002
Externally published	Yes

Keywords

Autotaxin
Cdc42
Focal adhesion kinase
Rac1

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(02)03698-0

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title = "Cdc42 and Rac1 are necessary for autotaxin-induced tumor cell motility in A2058 melanoma cells",

abstract = "Autotaxin (ATX) is a strong motogen that can increase invasiveness and angiogenesis. In the present study, we investigated the signal transduction mechanism of ATX-induced tumor cell motility. Unlike N19RhoA expressing cells, the cells expressing N17Cdc42 or N17Rac1 showed reduced motility against ATX. ATX activated Cdc42 and Rac1 and increased complex formation between these small G proteins and p21-activated kinase (PAK). Furthermore, ATX phosphorylated focal adhesion kinase (FAK) that was not shown in cells expressing dominant negative mutants of Cdc42 or Rac1. Collectively, these data strongly indicate that Cdc42 and Rac1 are essential for ATX-induced tumor cell motility in A2058 melanoma cells, and that PAK and FAK might be also involved in the process.",

keywords = "Autotaxin, Cdc42, Focal adhesion kinase, Rac1",

author = "Jung, {In Duk} and Jangsoon Lee and Yun, {Seong Young} and Park, {Chang Gyo} and Choi, {Wahn Soo} and Lee, {Hyang Woo} and Choi, {Oksoon H.} and Han, {Jeung Whan} and Lee, {Hoi Young}",

note = "Funding Information: This work was supported by a Korea Research Foundation Grant (KRF-2001-002-F00088). We would like to thank Dr. Seung-Kil Park for N19RhoA, and Dr. Dongeun Park for GST-PBD.",

year = "2002",

month = dec,

day = "18",

doi = "10.1016/S0014-5793(02)03698-0",

language = "English (US)",

volume = "532",

pages = "351--356",

journal = "FEBS Letters",

issn = "0014-5793",

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number = "3",

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TY - JOUR

T1 - Cdc42 and Rac1 are necessary for autotaxin-induced tumor cell motility in A2058 melanoma cells

AU - Jung, In Duk

AU - Lee, Jangsoon

AU - Yun, Seong Young

AU - Park, Chang Gyo

AU - Choi, Wahn Soo

AU - Lee, Hyang Woo

AU - Choi, Oksoon H.

AU - Han, Jeung Whan

AU - Lee, Hoi Young

N1 - Funding Information: This work was supported by a Korea Research Foundation Grant (KRF-2001-002-F00088). We would like to thank Dr. Seung-Kil Park for N19RhoA, and Dr. Dongeun Park for GST-PBD.

PY - 2002/12/18

Y1 - 2002/12/18

N2 - Autotaxin (ATX) is a strong motogen that can increase invasiveness and angiogenesis. In the present study, we investigated the signal transduction mechanism of ATX-induced tumor cell motility. Unlike N19RhoA expressing cells, the cells expressing N17Cdc42 or N17Rac1 showed reduced motility against ATX. ATX activated Cdc42 and Rac1 and increased complex formation between these small G proteins and p21-activated kinase (PAK). Furthermore, ATX phosphorylated focal adhesion kinase (FAK) that was not shown in cells expressing dominant negative mutants of Cdc42 or Rac1. Collectively, these data strongly indicate that Cdc42 and Rac1 are essential for ATX-induced tumor cell motility in A2058 melanoma cells, and that PAK and FAK might be also involved in the process.

AB - Autotaxin (ATX) is a strong motogen that can increase invasiveness and angiogenesis. In the present study, we investigated the signal transduction mechanism of ATX-induced tumor cell motility. Unlike N19RhoA expressing cells, the cells expressing N17Cdc42 or N17Rac1 showed reduced motility against ATX. ATX activated Cdc42 and Rac1 and increased complex formation between these small G proteins and p21-activated kinase (PAK). Furthermore, ATX phosphorylated focal adhesion kinase (FAK) that was not shown in cells expressing dominant negative mutants of Cdc42 or Rac1. Collectively, these data strongly indicate that Cdc42 and Rac1 are essential for ATX-induced tumor cell motility in A2058 melanoma cells, and that PAK and FAK might be also involved in the process.

KW - Autotaxin

KW - Cdc42

KW - Focal adhesion kinase

KW - Rac1

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U2 - 10.1016/S0014-5793(02)03698-0

DO - 10.1016/S0014-5793(02)03698-0

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AN - SCOPUS:0037132539

VL - 532

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EP - 356

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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ER -

Cdc42 and Rac1 are necessary for autotaxin-induced tumor cell motility in A2058 melanoma cells

Abstract

Keywords

ASJC Scopus subject areas

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