Cdc42 and Rac1 are necessary for autotaxin-induced tumor cell motility in A2058 melanoma cells

In Duk Jung, Jangsoon Lee, Seong Young Yun, Chang Gyo Park, Wahn Soo Choi, Hyang Woo Lee, Oksoon H. Choi, Jeung Whan Han, Hoi Young Lee

Research output: Contribution to journalArticle

Abstract

Autotaxin (ATX) is a strong motogen that can increase invasiveness and angiogenesis. In the present study, we investigated the signal transduction mechanism of ATX-induced tumor cell motility. Unlike N19RhoA expressing cells, the cells expressing N17Cdc42 or N17Rac1 showed reduced motility against ATX. ATX activated Cdc42 and Rac1 and increased complex formation between these small G proteins and p21-activated kinase (PAK). Furthermore, ATX phosphorylated focal adhesion kinase (FAK) that was not shown in cells expressing dominant negative mutants of Cdc42 or Rac1. Collectively, these data strongly indicate that Cdc42 and Rac1 are essential for ATX-induced tumor cell motility in A2058 melanoma cells, and that PAK and FAK might be also involved in the process.

Original languageEnglish (US)
Pages (from-to)351-356
Number of pages6
JournalFEBS Letters
Volume532
Issue number3
DOIs
StatePublished - Dec 18 2002
Externally publishedYes

Fingerprint

Cell Movement
Tumors
Melanoma
Cells
p21-Activated Kinases
Focal Adhesion Protein-Tyrosine Kinases
Neoplasms
Signal transduction
Monomeric GTP-Binding Proteins
Signal Transduction

Keywords

  • Autotaxin
  • Cdc42
  • Focal adhesion kinase
  • Rac1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Jung, I. D., Lee, J., Yun, S. Y., Park, C. G., Choi, W. S., Lee, H. W., ... Lee, H. Y. (2002). Cdc42 and Rac1 are necessary for autotaxin-induced tumor cell motility in A2058 melanoma cells. FEBS Letters, 532(3), 351-356. https://doi.org/10.1016/S0014-5793(02)03698-0

Cdc42 and Rac1 are necessary for autotaxin-induced tumor cell motility in A2058 melanoma cells. / Jung, In Duk; Lee, Jangsoon; Yun, Seong Young; Park, Chang Gyo; Choi, Wahn Soo; Lee, Hyang Woo; Choi, Oksoon H.; Han, Jeung Whan; Lee, Hoi Young.

In: FEBS Letters, Vol. 532, No. 3, 18.12.2002, p. 351-356.

Research output: Contribution to journalArticle

Jung, ID, Lee, J, Yun, SY, Park, CG, Choi, WS, Lee, HW, Choi, OH, Han, JW & Lee, HY 2002, 'Cdc42 and Rac1 are necessary for autotaxin-induced tumor cell motility in A2058 melanoma cells', FEBS Letters, vol. 532, no. 3, pp. 351-356. https://doi.org/10.1016/S0014-5793(02)03698-0
Jung, In Duk ; Lee, Jangsoon ; Yun, Seong Young ; Park, Chang Gyo ; Choi, Wahn Soo ; Lee, Hyang Woo ; Choi, Oksoon H. ; Han, Jeung Whan ; Lee, Hoi Young. / Cdc42 and Rac1 are necessary for autotaxin-induced tumor cell motility in A2058 melanoma cells. In: FEBS Letters. 2002 ; Vol. 532, No. 3. pp. 351-356.
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AU - Choi, Wahn Soo

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AB - Autotaxin (ATX) is a strong motogen that can increase invasiveness and angiogenesis. In the present study, we investigated the signal transduction mechanism of ATX-induced tumor cell motility. Unlike N19RhoA expressing cells, the cells expressing N17Cdc42 or N17Rac1 showed reduced motility against ATX. ATX activated Cdc42 and Rac1 and increased complex formation between these small G proteins and p21-activated kinase (PAK). Furthermore, ATX phosphorylated focal adhesion kinase (FAK) that was not shown in cells expressing dominant negative mutants of Cdc42 or Rac1. Collectively, these data strongly indicate that Cdc42 and Rac1 are essential for ATX-induced tumor cell motility in A2058 melanoma cells, and that PAK and FAK might be also involved in the process.

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