TY - JOUR
T1 - CD8 expression alters the fine specificity of an alloreactive MHC class l-specific T hybridoma
AU - Blok, Rozanne
AU - Margulies, David H.
AU - Pease, Larry
AU - Ribaudo, Randall K.
AU - Schneck, Jonathan
AU - Mccluskey, James
N1 - Funding Information:
This work was supported in part by grants from the NHMRC, Australia, and Keflion Diabetes Foundation. R.B. was supported by a Monash University Postgraduate Scholarship, R.K.R. is supported by the Cancer Research Institute and the Multiple Sclerosis Society, and J.S. is an American Arthritis Investigator and a Harry Weaver Neuroscience Scholar. We are grateful to Dr M. E. Dorf for the gift of the T hybridoma HTB-157.7.
PY - 1992/4
Y1 - 1992/4
N2 - The influence of CD8 on the fine specificity of MHC class l-restrlcted T cell allorecognition was evaluated by comparing the reactivity of CD8- and CD8-transfected forms of an allospecific, H-2Kb-restricted T hybridoma. The CD8- T hybridoma responded to cells expressing H-2Kb, H-2Kbm6and the individual H-2Kb-bm10back mutations 165V-M, 173K-E, and 174N-L. Under the same conditions the CD8- T hybridoma responded poorly or not at all to cells expressing H-2kbm10, H-2Kbm8, the individual H-2Kbm1010 back mutants 163T-A and 167W-S, and the Individual H-2Kb-bm8back mutations 22Y-F and 24E-S. In contrast, T hybridoma cells expressing high levels of CD8 reacted strongly with antigen presenting cells (APC) expressing H-2Kb and H-2Kbm66 molecules, as well as APC expressing H-2Kbm10 (weakly), H-2Kb-bm8 and all five individual H-2Kb-bm10 and the two H-2Kb-bm8 back mutants 22Y↑F and 24E↑S. The mutations which distinguish the T cell recognition of both H-2Kbm10 and H=-2Kbm8 from H-2Kb are predicted to control the interaction of these class I molecules with antlgenic peptides In the binding site, Implying an important role for peptide antigen In T cell allorecognition. Nonetheless, CD8 expression by the H-2Kb-restricted T cells conferred novel or enhanced alloreactivlty with cells expressing H-2Kbm10, H-2Kbm8, and each of the individual H-2Kbm10 and H-2Kb-bm8 back mutants. These findings reflect an Important role for CD8 in influencing the fine specificity of MHC class I recognition by T cells and may Indicate a limited structural role for peptide antigen in defining the llgand recognized by these alloreactive T cells
AB - The influence of CD8 on the fine specificity of MHC class l-restrlcted T cell allorecognition was evaluated by comparing the reactivity of CD8- and CD8-transfected forms of an allospecific, H-2Kb-restricted T hybridoma. The CD8- T hybridoma responded to cells expressing H-2Kb, H-2Kbm6and the individual H-2Kb-bm10back mutations 165V-M, 173K-E, and 174N-L. Under the same conditions the CD8- T hybridoma responded poorly or not at all to cells expressing H-2kbm10, H-2Kbm8, the individual H-2Kbm1010 back mutants 163T-A and 167W-S, and the Individual H-2Kb-bm8back mutations 22Y-F and 24E-S. In contrast, T hybridoma cells expressing high levels of CD8 reacted strongly with antigen presenting cells (APC) expressing H-2Kb and H-2Kbm66 molecules, as well as APC expressing H-2Kbm10 (weakly), H-2Kb-bm8 and all five individual H-2Kb-bm10 and the two H-2Kb-bm8 back mutants 22Y↑F and 24E↑S. The mutations which distinguish the T cell recognition of both H-2Kbm10 and H=-2Kbm8 from H-2Kb are predicted to control the interaction of these class I molecules with antlgenic peptides In the binding site, Implying an important role for peptide antigen In T cell allorecognition. Nonetheless, CD8 expression by the H-2Kb-restricted T cells conferred novel or enhanced alloreactivlty with cells expressing H-2Kbm10, H-2Kbm8, and each of the individual H-2Kbm10 and H-2Kb-bm8 back mutants. These findings reflect an Important role for CD8 in influencing the fine specificity of MHC class I recognition by T cells and may Indicate a limited structural role for peptide antigen in defining the llgand recognized by these alloreactive T cells
KW - Allorecognition
KW - Antigen recognition
KW - Co-receptor
KW - T cell activation
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U2 - 10.1093/intimm/4.4.455
DO - 10.1093/intimm/4.4.455
M3 - Article
C2 - 1591216
AN - SCOPUS:0026572709
SN - 0953-8178
VL - 4
SP - 455
EP - 466
JO - International Immunology
JF - International Immunology
IS - 4
ER -