CD8 expression alters the fine specificity of an alloreactive MHC class l-specific T hybridoma

Rozanne Blok, David H. Margulies, Larry Pease, Randall K. Ribaudo, Jonathan Schneck, James Mccluskey

Research output: Contribution to journalArticle

Abstract

The influence of CD8 on the fine specificity of MHC class l-restrlcted T cell allorecognition was evaluated by comparing the reactivity of CD8- and CD8-transfected forms of an allospecific, H-2Kb-restricted T hybridoma. The CD8- T hybridoma responded to cells expressing H-2Kb, H-2Kbm6and the individual H-2Kb-bm10back mutations 165V-M, 173K-E, and 174N-L. Under the same conditions the CD8- T hybridoma responded poorly or not at all to cells expressing H-2kbm10, H-2Kbm8, the individual H-2Kbm1010 back mutants 163T-A and 167W-S, and the Individual H-2Kb-bm8back mutations 22Y-F and 24E-S. In contrast, T hybridoma cells expressing high levels of CD8 reacted strongly with antigen presenting cells (APC) expressing H-2Kb and H-2Kbm66 molecules, as well as APC expressing H-2Kbm10 (weakly), H-2Kb-bm8 and all five individual H-2Kb-bm10 and the two H-2Kb-bm8 back mutants 22Y↑F and 24E↑S. The mutations which distinguish the T cell recognition of both H-2Kbm10 and H=-2Kbm8 from H-2Kb are predicted to control the interaction of these class I molecules with antlgenic peptides In the binding site, Implying an important role for peptide antigen In T cell allorecognition. Nonetheless, CD8 expression by the H-2Kb-restricted T cells conferred novel or enhanced alloreactivlty with cells expressing H-2Kbm10, H-2Kbm8, and each of the individual H-2Kbm10 and H-2Kb-bm8 back mutants. These findings reflect an Important role for CD8 in influencing the fine specificity of MHC class I recognition by T cells and may Indicate a limited structural role for peptide antigen in defining the llgand recognized by these alloreactive T cells

Original languageEnglish (US)
Pages (from-to)455-466
Number of pages12
JournalInternational Immunology
Volume4
Issue number4
DOIs
StatePublished - Apr 1 1992

Keywords

  • Allorecognition
  • Antigen recognition
  • Co-receptor
  • T cell activation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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