TY - JOUR
T1 - CD68 acts as a major gateway for malaria sporozoite liver infection
AU - Cha, Sung Jae
AU - Park, Kiwon
AU - Srinivasan, Prakash
AU - Schindler, Christian W.
AU - Van Rooijen, Nico
AU - Stins, Monique
AU - Jacobs-Lorena, Marcelo
N1 - Publisher Copyright:
© 2015 Cha et al.
PY - 2015/8/24
Y1 - 2015/8/24
N2 - After being delivered by the bite from an infected mosquito, Plasmodium sporozoites enter the blood circulation and infect the liver. Previous evidence suggests that Kupffer cells, a macrophage-like component of the liver blood vessel lining, are traversed by sporozoites to initiate liver invasion. However, the molecular determinants of sporozoite-Kupffer cell interactions are unknown. Understanding the molecular basis for this specific recognition may lead to novel therapeutic strategies to control malaria. Using a phage display library screen, we identified a peptide, P39, that strongly binds to the Kupffer cell surface and, importantly, inhibits sporozoite Kupffer cell entry. Furthermore, we determined that P39 binds to CD68, a putative receptor for sporozoite invasion of Kupffer cells that acts as a gateway for malaria infection of the liver.
AB - After being delivered by the bite from an infected mosquito, Plasmodium sporozoites enter the blood circulation and infect the liver. Previous evidence suggests that Kupffer cells, a macrophage-like component of the liver blood vessel lining, are traversed by sporozoites to initiate liver invasion. However, the molecular determinants of sporozoite-Kupffer cell interactions are unknown. Understanding the molecular basis for this specific recognition may lead to novel therapeutic strategies to control malaria. Using a phage display library screen, we identified a peptide, P39, that strongly binds to the Kupffer cell surface and, importantly, inhibits sporozoite Kupffer cell entry. Furthermore, we determined that P39 binds to CD68, a putative receptor for sporozoite invasion of Kupffer cells that acts as a gateway for malaria infection of the liver.
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U2 - 10.1084/jem.20110575
DO - 10.1084/jem.20110575
M3 - Article
C2 - 26216124
AN - SCOPUS:84959527807
SN - 0022-1007
VL - 212
SP - 1391
EP - 1403
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 9
ER -