CD4+ T-cells from mice immunized to syngeneic sarcomas recognize distinct, non-shared tumor antigens

Peter A. Cohen, Philip J. Cohen, Steven A. Rosenberg, James J. Mulé

Research output: Contribution to journalArticle

Abstract

We have utilized a newly developed culture system to study the properties of antitumor CD4+ T-cells relevant to the rejection of syngeneic methylcholanthrene sarcomas. Fresh syngeneic dendritic cells prepared from spleen, then pulsed with crude lysates of methylcholanthrene sarcomas, evoke antigen-specific proliferation by CD4+ but not by CD8+ T-cells from tumor- immune mice. Unfractionated splenocytes display similar antigen presenting capacity if they are not irradiated before the pulse with tumor lysate. CD4+ T-cells from mice immunized to individual methylcholanthrene sarcomas proliferate cross-reactively to dendritic cells pulsed with fresh tumor digests, but not to dendritic cells pulsed with cultured tumor cells. This apparent shared recognition of sarcoma lysates was demonstrated to be a result of sensitization to bacterial collagenase during the immunization procedure. Therefore, the murine CD4+ T-cell response to tumor immunization is similar to the CD8+ response in that sensitization occurs predominantly to tumor specific transplantation antigens rather than to shared tumor antigens. Strategies to avoid artefactual tumor cross-recognition by CD4+ T- cells are discussed.

Original languageEnglish (US)
Pages (from-to)1055-1058
Number of pages4
JournalCancer Research
Volume54
Issue number4
StatePublished - Feb 15 1994
Externally publishedYes

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Neoplasm Antigens
Sarcoma
Methylcholanthrene
T-Lymphocytes
Dendritic Cells
Neoplasms
Immunization
Cultured Tumor Cells
Antigens
Collagenases
Spleen

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Cohen, P. A., Cohen, P. J., Rosenberg, S. A., & Mulé, J. J. (1994). CD4+ T-cells from mice immunized to syngeneic sarcomas recognize distinct, non-shared tumor antigens. Cancer Research, 54(4), 1055-1058.

CD4+ T-cells from mice immunized to syngeneic sarcomas recognize distinct, non-shared tumor antigens. / Cohen, Peter A.; Cohen, Philip J.; Rosenberg, Steven A.; Mulé, James J.

In: Cancer Research, Vol. 54, No. 4, 15.02.1994, p. 1055-1058.

Research output: Contribution to journalArticle

Cohen, PA, Cohen, PJ, Rosenberg, SA & Mulé, JJ 1994, 'CD4+ T-cells from mice immunized to syngeneic sarcomas recognize distinct, non-shared tumor antigens', Cancer Research, vol. 54, no. 4, pp. 1055-1058.
Cohen, Peter A. ; Cohen, Philip J. ; Rosenberg, Steven A. ; Mulé, James J. / CD4+ T-cells from mice immunized to syngeneic sarcomas recognize distinct, non-shared tumor antigens. In: Cancer Research. 1994 ; Vol. 54, No. 4. pp. 1055-1058.
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