TY - JOUR
T1 - CD4+ T cell responses to SSX-4 in melanoma patients
AU - Ayyoub, Maha
AU - Merlo, Andrea
AU - Hesdorffer, Charles S.
AU - Rimoldi, Donata
AU - Speiser, Daniel
AU - Cerottini, Jean Charles
AU - Chen, Yao Tseng
AU - Old, Lloyd J.
AU - Stevanovic, Stefan
AU - Valmori, Danila
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2005/4/15
Y1 - 2005/4/15
N2 - Genes of the synovial sarcoma X breakpoint (SSX) family are expressed in different human tumors, including melanomas, but not in adult somatic tissues. Because of their specific expression at the tumor site, SSX-encoded Ags are potential targets for anticancer immunotherapy. In this study, we have analyzed CD4+ T cell responses directed against the Ag encoded by SSX-4. Upon in vitro stimulation of PBMC from four melanoma patients bearing Ag-expressing tumors with a pool of long peptides spanning the protein sequence, we detected and isolated SSX-4-specific CD4+ T cells recognizing several distinct antigenic sequences, mostly restricted by frequently expressed HLA class II alleles. The majority of the identified sequences were located within the Krüppel-associated box domain in the N-terminal region of the protein, indicating a high potential immunogenicity of this region. Together our data document the existence of CD4+ T cells specific for multiple SSX-4 derived sequences in circulating lymphocytes from melanoma patients and encourage further studies to assess the impact of SSX-4-specific T cell responses on disease evolution in cancer patients.
AB - Genes of the synovial sarcoma X breakpoint (SSX) family are expressed in different human tumors, including melanomas, but not in adult somatic tissues. Because of their specific expression at the tumor site, SSX-encoded Ags are potential targets for anticancer immunotherapy. In this study, we have analyzed CD4+ T cell responses directed against the Ag encoded by SSX-4. Upon in vitro stimulation of PBMC from four melanoma patients bearing Ag-expressing tumors with a pool of long peptides spanning the protein sequence, we detected and isolated SSX-4-specific CD4+ T cells recognizing several distinct antigenic sequences, mostly restricted by frequently expressed HLA class II alleles. The majority of the identified sequences were located within the Krüppel-associated box domain in the N-terminal region of the protein, indicating a high potential immunogenicity of this region. Together our data document the existence of CD4+ T cells specific for multiple SSX-4 derived sequences in circulating lymphocytes from melanoma patients and encourage further studies to assess the impact of SSX-4-specific T cell responses on disease evolution in cancer patients.
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U2 - 10.4049/jimmunol.174.8.5092
DO - 10.4049/jimmunol.174.8.5092
M3 - Article
C2 - 15814740
AN - SCOPUS:20144387723
SN - 0022-1767
VL - 174
SP - 5092
EP - 5099
JO - Journal of Immunology
JF - Journal of Immunology
IS - 8
ER -