CD47 signaling regulates the immunosuppressive activity of VEGF in T cells

Sukhbir Kaur, Tiffany Chang, Satya P. Singh, Langston Lim, Poonam Mannan, Susan H. Garfield, Michael L. Pendrak, David R. Soto-Pantoja, Avi Rosenberg, Shelly Jin, David D. Roberts

Research output: Contribution to journalArticle

Abstract

Thrombospondin-1 (TSP1) inhibits angiogenesis, in part, by interacting with the ubiquitous cell-surface receptor CD47. In endothelial cells, CD47 interacts directly with vascular endothelial growth factor receptor (VEGFR)-2, and TSP1 inhibits VEGFR2 phosphorylation and signaling by disrupting this association.We show that CD47 similarly associates with and regulates VEGFR2 in T cells. TSP1 inhibits phosphorylation of VEGFR2 and its downstream target Src in wild type but not in CD47-deficient human Jurkat and primary murine T cells. VEGFR2 signaling inhibits proliferation and TCR signaling in wild type T cells. However, ligation of CD47 by TSP1 or loss of CD47 expression reverses some inhibitory effects of VEGF on proliferation and T cell activation. We further found that VEGF and VEGFR2 expression are upregulated in CD47-deficient murine CD4+ and human Jurkat T cells, and the resulting autocrine VEGFR2 signaling enhances proliferation and some TCR responses in the absence of CD47. Thus, CD47 signaling modulates the ability of VEGF to regulate proliferation and TCR signaling, and autocrine production of VEGF by T cells contributes to this regulation. This provides a mechanism to understand the context-dependent effects of TSP1 and VEGF on T cell activation, and reveals an important role for CD47 signaling in regulating T cell production of the major angiogenic factor VEGF.

Original languageEnglish (US)
Pages (from-to)3914-3924
Number of pages11
JournalJournal of Immunology
Volume193
Issue number8
DOIs
StatePublished - Oct 15 2014
Externally publishedYes

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Immunosuppressive Agents
Vascular Endothelial Growth Factor A
Thrombospondin 1
T-Lymphocytes
Autocrine Communication
Phosphorylation
Vascular Endothelial Growth Factor Receptor-2
Jurkat Cells
Angiogenesis Inducing Agents
Cell Surface Receptors
Ligation
Endothelial Cells

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Kaur, S., Chang, T., Singh, S. P., Lim, L., Mannan, P., Garfield, S. H., ... Roberts, D. D. (2014). CD47 signaling regulates the immunosuppressive activity of VEGF in T cells. Journal of Immunology, 193(8), 3914-3924. https://doi.org/10.4049/jimmunol.1303116

CD47 signaling regulates the immunosuppressive activity of VEGF in T cells. / Kaur, Sukhbir; Chang, Tiffany; Singh, Satya P.; Lim, Langston; Mannan, Poonam; Garfield, Susan H.; Pendrak, Michael L.; Soto-Pantoja, David R.; Rosenberg, Avi; Jin, Shelly; Roberts, David D.

In: Journal of Immunology, Vol. 193, No. 8, 15.10.2014, p. 3914-3924.

Research output: Contribution to journalArticle

Kaur, S, Chang, T, Singh, SP, Lim, L, Mannan, P, Garfield, SH, Pendrak, ML, Soto-Pantoja, DR, Rosenberg, A, Jin, S & Roberts, DD 2014, 'CD47 signaling regulates the immunosuppressive activity of VEGF in T cells', Journal of Immunology, vol. 193, no. 8, pp. 3914-3924. https://doi.org/10.4049/jimmunol.1303116
Kaur S, Chang T, Singh SP, Lim L, Mannan P, Garfield SH et al. CD47 signaling regulates the immunosuppressive activity of VEGF in T cells. Journal of Immunology. 2014 Oct 15;193(8):3914-3924. https://doi.org/10.4049/jimmunol.1303116
Kaur, Sukhbir ; Chang, Tiffany ; Singh, Satya P. ; Lim, Langston ; Mannan, Poonam ; Garfield, Susan H. ; Pendrak, Michael L. ; Soto-Pantoja, David R. ; Rosenberg, Avi ; Jin, Shelly ; Roberts, David D. / CD47 signaling regulates the immunosuppressive activity of VEGF in T cells. In: Journal of Immunology. 2014 ; Vol. 193, No. 8. pp. 3914-3924.
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