CD4+ T cell responses to SSX-4 in melanoma patients

Maha Ayyoub, Andrea Merlo, Charles S. Hesdorffer, Donata Rimoldi, Daniel Speiser, Jean Charles Cerottini, Yao Tseng Chen, Lloyd J. Old, Stefan Stevanovic, Danila Valmori

Research output: Contribution to journalArticle

Abstract

Genes of the synovial sarcoma X breakpoint (SSX) family are expressed in different human tumors, including melanomas, but not in adult somatic tissues. Because of their specific expression at the tumor site, SSX-encoded Ags are potential targets for anticancer immunotherapy. In this study, we have analyzed CD4+ T cell responses directed against the Ag encoded by SSX-4. Upon in vitro stimulation of PBMC from four melanoma patients bearing Ag-expressing tumors with a pool of long peptides spanning the protein sequence, we detected and isolated SSX-4-specific CD4+ T cells recognizing several distinct antigenic sequences, mostly restricted by frequently expressed HLA class II alleles. The majority of the identified sequences were located within the Krüppel-associated box domain in the N-terminal region of the protein, indicating a high potential immunogenicity of this region. Together our data document the existence of CD4+ T cells specific for multiple SSX-4 derived sequences in circulating lymphocytes from melanoma patients and encourage further studies to assess the impact of SSX-4-specific T cell responses on disease evolution in cancer patients.

Original languageEnglish (US)
Pages (from-to)5092-5099
Number of pages8
JournalJournal of Immunology
Volume174
Issue number8
StatePublished - Apr 15 2005
Externally publishedYes

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Synovial Sarcoma
Melanoma
T-Lymphocytes
Neoplasms
Immunotherapy
Proteins
Alleles
Lymphocytes
Peptides
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Ayyoub, M., Merlo, A., Hesdorffer, C. S., Rimoldi, D., Speiser, D., Cerottini, J. C., ... Valmori, D. (2005). CD4+ T cell responses to SSX-4 in melanoma patients. Journal of Immunology, 174(8), 5092-5099.

CD4+ T cell responses to SSX-4 in melanoma patients. / Ayyoub, Maha; Merlo, Andrea; Hesdorffer, Charles S.; Rimoldi, Donata; Speiser, Daniel; Cerottini, Jean Charles; Chen, Yao Tseng; Old, Lloyd J.; Stevanovic, Stefan; Valmori, Danila.

In: Journal of Immunology, Vol. 174, No. 8, 15.04.2005, p. 5092-5099.

Research output: Contribution to journalArticle

Ayyoub, M, Merlo, A, Hesdorffer, CS, Rimoldi, D, Speiser, D, Cerottini, JC, Chen, YT, Old, LJ, Stevanovic, S & Valmori, D 2005, 'CD4+ T cell responses to SSX-4 in melanoma patients', Journal of Immunology, vol. 174, no. 8, pp. 5092-5099.
Ayyoub M, Merlo A, Hesdorffer CS, Rimoldi D, Speiser D, Cerottini JC et al. CD4+ T cell responses to SSX-4 in melanoma patients. Journal of Immunology. 2005 Apr 15;174(8):5092-5099.
Ayyoub, Maha ; Merlo, Andrea ; Hesdorffer, Charles S. ; Rimoldi, Donata ; Speiser, Daniel ; Cerottini, Jean Charles ; Chen, Yao Tseng ; Old, Lloyd J. ; Stevanovic, Stefan ; Valmori, Danila. / CD4+ T cell responses to SSX-4 in melanoma patients. In: Journal of Immunology. 2005 ; Vol. 174, No. 8. pp. 5092-5099.
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