CD34 antigen expression of acute myeloid leukemia‐colony forming cells

F. Silvestri, S. Banavali, M. Yin, B. Hulette, V. Gopal, C. I. Civin, M. Baccarani, H. D. Preisler

Research output: Contribution to journalArticle

Abstract

Hematopoietic The International Journal of Cell Cloning express the CD34 antigen on their surface. Their proportion in a harvest can provide a quick and reliable measure of the hemopoietic efficiency of the harvest. In ANLL a variable proportion of blast cells express the same antigen: the aim of our study was to investigate whether clonogenic ANLL blast cells are CD34+. Seven peripheral blood samples of patients with ANLL were studied. From these, CD34+ cells were isolated using My10 antibody, immunomagnetic beads and chymopapain. About 60% of CD34+ cells were recovered in the positive fraction, while the negative fraction contained less than 1% CD34+ cells. Cloning these fractions resulted in an almost complete absence of CFU in the negative fraction. This study shows that leukemia‐colony forming cells are, as normal CFU, CD34+ and that they represent only a small fraction (less than 1%) of the CD34+ leukemic population.

Original languageEnglish (US)
Pages (from-to)98-100
Number of pages3
JournalThe International Journal of Cell Cloning
Volume10
Issue number1 S
DOIs
StatePublished - 1992

Fingerprint

CD34 Antigens
Acute Myeloid Leukemia
Organism Cloning
Chymopapain
Antigens
Antibodies

Keywords

  • acute myeloid leukemia‐colony forming cells
  • CD34 Antigen
  • peripheral blood stem cell transplantation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Silvestri, F., Banavali, S., Yin, M., Hulette, B., Gopal, V., Civin, C. I., ... Preisler, H. D. (1992). CD34 antigen expression of acute myeloid leukemia‐colony forming cells. The International Journal of Cell Cloning, 10(1 S), 98-100. https://doi.org/10.1002/stem.5530100733

CD34 antigen expression of acute myeloid leukemia‐colony forming cells. / Silvestri, F.; Banavali, S.; Yin, M.; Hulette, B.; Gopal, V.; Civin, C. I.; Baccarani, M.; Preisler, H. D.

In: The International Journal of Cell Cloning, Vol. 10, No. 1 S, 1992, p. 98-100.

Research output: Contribution to journalArticle

Silvestri, F, Banavali, S, Yin, M, Hulette, B, Gopal, V, Civin, CI, Baccarani, M & Preisler, HD 1992, 'CD34 antigen expression of acute myeloid leukemia‐colony forming cells', The International Journal of Cell Cloning, vol. 10, no. 1 S, pp. 98-100. https://doi.org/10.1002/stem.5530100733
Silvestri, F. ; Banavali, S. ; Yin, M. ; Hulette, B. ; Gopal, V. ; Civin, C. I. ; Baccarani, M. ; Preisler, H. D. / CD34 antigen expression of acute myeloid leukemia‐colony forming cells. In: The International Journal of Cell Cloning. 1992 ; Vol. 10, No. 1 S. pp. 98-100.
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