Abstract
Hematopoietic The International Journal of Cell Cloning express the CD34 antigen on their surface. Their proportion in a harvest can provide a quick and reliable measure of the hemopoietic efficiency of the harvest. In ANLL a variable proportion of blast cells express the same antigen: the aim of our study was to investigate whether clonogenic ANLL blast cells are CD34+. Seven peripheral blood samples of patients with ANLL were studied. From these, CD34+ cells were isolated using My10 antibody, immunomagnetic beads and chymopapain. About 60% of CD34+ cells were recovered in the positive fraction, while the negative fraction contained less than 1% CD34+ cells. Cloning these fractions resulted in an almost complete absence of CFU in the negative fraction. This study shows that leukemia‐colony forming cells are, as normal CFU, CD34+ and that they represent only a small fraction (less than 1%) of the CD34+ leukemic population.
Original language | English (US) |
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Pages (from-to) | 98-100 |
Number of pages | 3 |
Journal | The International Journal of Cell Cloning |
Volume | 10 |
Issue number | 1 S |
DOIs | |
State | Published - 1992 |
Keywords
- acute myeloid leukemia‐colony forming cells
- CD34 Antigen
- peripheral blood stem cell transplantation
ASJC Scopus subject areas
- Cell Biology