CD34 antigen expression of acute myeloid leukemia‐colony forming cells

F. Silvestri, S. Banavali, M. Yin, B. Hulette, V. Gopal, C. I. Civin, M. Baccarani, H. D. Preisler

Research output: Contribution to journalArticlepeer-review

Abstract

Hematopoietic The International Journal of Cell Cloning express the CD34 antigen on their surface. Their proportion in a harvest can provide a quick and reliable measure of the hemopoietic efficiency of the harvest. In ANLL a variable proportion of blast cells express the same antigen: the aim of our study was to investigate whether clonogenic ANLL blast cells are CD34+. Seven peripheral blood samples of patients with ANLL were studied. From these, CD34+ cells were isolated using My10 antibody, immunomagnetic beads and chymopapain. About 60% of CD34+ cells were recovered in the positive fraction, while the negative fraction contained less than 1% CD34+ cells. Cloning these fractions resulted in an almost complete absence of CFU in the negative fraction. This study shows that leukemia‐colony forming cells are, as normal CFU, CD34+ and that they represent only a small fraction (less than 1%) of the CD34+ leukemic population.

Original languageEnglish (US)
Pages (from-to)98-100
Number of pages3
JournalThe International Journal of Cell Cloning
Volume10
Issue number1 S
DOIs
StatePublished - 1992

Keywords

  • acute myeloid leukemia‐colony forming cells
  • CD34 Antigen
  • peripheral blood stem cell transplantation

ASJC Scopus subject areas

  • Cell Biology

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