CD13/Aminopeptidase N overexpression by basic fibroblast growth factor mediates enhanced invasiveness of 1F6 human melanoma cells

D. Fontijn, M. C A Duyndam, M. P A Van Berkel, Y. Yuana, L. H. Shapiro, H. M. Pinedo, H. J. Broxterman, E. Boven

Research output: Contribution to journalArticle

Abstract

CD13/Aminopeptidase N (CD13) is known to play an important role in tumour cell invasion. We examined whether basic fibroblast growth factor (bFGF) is involved in the regulation of CD13 expression in human melanoma cells. 1F6 human melanoma cells were stably transfected with constructs encoding either the 18kDa (18kD) or all (ALL) bFGF isoform proteins. We observed highly increased CD13 mRNA and protein expression in the 1F6 clones regardless of the overexpression of either the 18kD or all isoform proteins. Neutral aminopeptidase activity was increased five-fold and could be inhibited by bestatin and the CD13-neutralising antibody WM15. The enhanced invasion through Matrigel, but not migration in a wound assay, was efficiently abrogated by both bestatin and WM15. Upregulation of CD13 expression was the result of increased epithelial and myeloid promoter activity up to 4.5-fold in 1F6-18kD and 1F6-ALL clones. Interestingly, in a panel of human melanoma cell lines, a significant correlation (r2=0.883, P

Original languageEnglish (US)
Pages (from-to)1627-1636
Number of pages10
JournalBritish Journal of Cancer
Volume94
Issue number11
DOIs
StatePublished - Jun 5 2006
Externally publishedYes

Fingerprint

CD13 Antigens
Fibroblast Growth Factor 2
Melanoma
Protein Isoforms
Clone Cells
Aminopeptidases
Neutralizing Antibodies
Up-Regulation
Cell Line
Messenger RNA
Wounds and Injuries
Neoplasms
Proteins
ubenimex

Keywords

  • bFGF
  • CD13
  • Invasion
  • Melanoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Fontijn, D., Duyndam, M. C. A., Van Berkel, M. P. A., Yuana, Y., Shapiro, L. H., Pinedo, H. M., ... Boven, E. (2006). CD13/Aminopeptidase N overexpression by basic fibroblast growth factor mediates enhanced invasiveness of 1F6 human melanoma cells. British Journal of Cancer, 94(11), 1627-1636. https://doi.org/10.1038/sj.bjc.6603157

CD13/Aminopeptidase N overexpression by basic fibroblast growth factor mediates enhanced invasiveness of 1F6 human melanoma cells. / Fontijn, D.; Duyndam, M. C A; Van Berkel, M. P A; Yuana, Y.; Shapiro, L. H.; Pinedo, H. M.; Broxterman, H. J.; Boven, E.

In: British Journal of Cancer, Vol. 94, No. 11, 05.06.2006, p. 1627-1636.

Research output: Contribution to journalArticle

Fontijn, D, Duyndam, MCA, Van Berkel, MPA, Yuana, Y, Shapiro, LH, Pinedo, HM, Broxterman, HJ & Boven, E 2006, 'CD13/Aminopeptidase N overexpression by basic fibroblast growth factor mediates enhanced invasiveness of 1F6 human melanoma cells', British Journal of Cancer, vol. 94, no. 11, pp. 1627-1636. https://doi.org/10.1038/sj.bjc.6603157
Fontijn, D. ; Duyndam, M. C A ; Van Berkel, M. P A ; Yuana, Y. ; Shapiro, L. H. ; Pinedo, H. M. ; Broxterman, H. J. ; Boven, E. / CD13/Aminopeptidase N overexpression by basic fibroblast growth factor mediates enhanced invasiveness of 1F6 human melanoma cells. In: British Journal of Cancer. 2006 ; Vol. 94, No. 11. pp. 1627-1636.
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