CD133/CD15 defines distinct cell subpopulations with differential in vitro clonogenic activity and stem cell-related gene expression profile in in vitro propagated glioblastoma multiforme-derived cell line with a PNET-like component

Ulf D. Kahlert, Noemi O. Bender, Donata Maciaczyk, Tomasz Bogiel, Eli E. Bar, Charles G Eberhart, Guido Nikkhah, Jaroslaw Maciaczyk

Research output: Contribution to journalArticle

Abstract

Glioblastoma multiforme (GBM), as many other solid tumours, contains a subpopulation of cells termed cancer stemlike cells responsible for the initiation and propagation of tumour growth. However, a unique immunophenotype/surface antigen composition for the clear identification of brain tumour stem cells (BTSC) has not yet been found. Here we report a novel code of cell surface markers for the identification of different cell subpopulations in neurospheres derived from a GBM with a primitive neuroectodermal tumour (PNET)-like component (GBM-PNET). These subgroups differ in their CD133/CD15 expression pattern and resemble cells with different stem-like genotype and developmental pathway activation levels. Strikingly, clonogenic analysis of cultures differentially expressing the investigated markers enabled the identification of distinct subpopulations of cells endowed with stem cell characteristics. High clonogenicity could be found in CD133 -/CD15- and CD133+/CD15+ but not in CD133-/CD15+ cells. Moreover, cell subpopulations with pronounced clonogenic growth were characterized by high expression of stem cell-related genes. Interestingly, these observations were unique for GBM-PNET and differed from ordinary GBM cultures derived from tumours lacking a PNET component. This work elucidates the complex molecular heterogeneity of in vitro propagated glioblastoma-derived cells and potentially contributes to the development of novel diagnostic modalities aiming at the identification of the brain tumour stem-like cell population in a subgroup of GBMs.

Original languageEnglish (US)
Pages (from-to)357-368
Number of pages12
JournalFolia Neuropathologica
Volume50
Issue number4
DOIs
StatePublished - 2012

Fingerprint

Primitive Neuroectodermal Tumors
Glioblastoma
Transcriptome
Stem Cells
Cell Line
Neoplastic Stem Cells
Brain Neoplasms
Neoplasms
In Vitro Techniques
Surface Antigens
Growth
Genotype

Keywords

  • Brain tumour stem-like cell
  • CD133
  • Clonogenicity
  • GBM
  • PNET

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology

Cite this

CD133/CD15 defines distinct cell subpopulations with differential in vitro clonogenic activity and stem cell-related gene expression profile in in vitro propagated glioblastoma multiforme-derived cell line with a PNET-like component. / Kahlert, Ulf D.; Bender, Noemi O.; Maciaczyk, Donata; Bogiel, Tomasz; Bar, Eli E.; Eberhart, Charles G; Nikkhah, Guido; Maciaczyk, Jaroslaw.

In: Folia Neuropathologica, Vol. 50, No. 4, 2012, p. 357-368.

Research output: Contribution to journalArticle

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