CD10 immunohistochemical staining enhances the histological detection of endometriosis

Clariss Potlog-Nahari, Andrew L. Feldman, Pamela Stratton, Deloris E. Koziol, James Segars, Maria J. Merino, Lynnette K. Nieman

Research output: Contribution to journalArticle

Abstract

Objective To determine whether the use of CD10 immunohistochemistry in addition to hematoxylin and eosin (H&E) staining would increase the sensitivity of surgically suspected endometriosis lesions. Design Retrospective cohort study. Setting Tertiary care government research hospital. Patient(s) Thirty-one women with chronic pelvic pain. Intervention(s) Immunohistochemical analysis for CD10 was performed on 108 possible endometriotic lesions and in the corresponding endometrial biopsy samples obtained during laparoscopy. When CD10 immunohistochemistry results were positive, the corresponding H&E section was reviewed to determine if the initial diagnosis should be revised. Main outcome measure(s) Histologic diagnosis of endometriosis by adjunctive use of CD10 immunohistochemistry in conjunction with H&E-stained specimens. Result(s) In endometrial stroma, CD10 was consistently present. Of the 70 specimens judged negative initially by H&E staining, CD10 staining led to the diagnosis of endometriosis in 11. The addition of CD10 immunohistochemistry detected more positive endometriosis lesions than H&E staining alone (45% vs. 35%). In three women with minimal endometriosis at surgery but initially negative histopathology, CD10 immunohistochemistry changed the histologic diagnosis to endometriosis. Conclusion(s) The adjunctive use of CD10 immunohistochemistry improves diagnostic sensitivity for endometriosis, especially for women with minimal disease.

Original languageEnglish (US)
Pages (from-to)86-92
Number of pages7
JournalFertility and Sterility
Volume82
Issue number1
DOIs
StatePublished - Jul 2004
Externally publishedYes

Fingerprint

Endometriosis
Staining and Labeling
Immunohistochemistry
Pelvic Pain
Tertiary Healthcare
Hematoxylin
Eosine Yellowish-(YS)
Chronic Pain
Laparoscopy
Cohort Studies
Retrospective Studies
Outcome Assessment (Health Care)
Biopsy
Research

Keywords

  • CD10
  • diagnostic test
  • endometriosis
  • hematoxylin and eosin
  • immunohistochemistry

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Potlog-Nahari, C., Feldman, A. L., Stratton, P., Koziol, D. E., Segars, J., Merino, M. J., & Nieman, L. K. (2004). CD10 immunohistochemical staining enhances the histological detection of endometriosis. Fertility and Sterility, 82(1), 86-92. https://doi.org/10.1016/j.fertnstert.2003.11.059

CD10 immunohistochemical staining enhances the histological detection of endometriosis. / Potlog-Nahari, Clariss; Feldman, Andrew L.; Stratton, Pamela; Koziol, Deloris E.; Segars, James; Merino, Maria J.; Nieman, Lynnette K.

In: Fertility and Sterility, Vol. 82, No. 1, 07.2004, p. 86-92.

Research output: Contribution to journalArticle

Potlog-Nahari, C, Feldman, AL, Stratton, P, Koziol, DE, Segars, J, Merino, MJ & Nieman, LK 2004, 'CD10 immunohistochemical staining enhances the histological detection of endometriosis', Fertility and Sterility, vol. 82, no. 1, pp. 86-92. https://doi.org/10.1016/j.fertnstert.2003.11.059
Potlog-Nahari, Clariss ; Feldman, Andrew L. ; Stratton, Pamela ; Koziol, Deloris E. ; Segars, James ; Merino, Maria J. ; Nieman, Lynnette K. / CD10 immunohistochemical staining enhances the histological detection of endometriosis. In: Fertility and Sterility. 2004 ; Vol. 82, No. 1. pp. 86-92.
@article{395cdcdc355b429c96ea1a2380a50f79,
title = "CD10 immunohistochemical staining enhances the histological detection of endometriosis",
abstract = "Objective To determine whether the use of CD10 immunohistochemistry in addition to hematoxylin and eosin (H&E) staining would increase the sensitivity of surgically suspected endometriosis lesions. Design Retrospective cohort study. Setting Tertiary care government research hospital. Patient(s) Thirty-one women with chronic pelvic pain. Intervention(s) Immunohistochemical analysis for CD10 was performed on 108 possible endometriotic lesions and in the corresponding endometrial biopsy samples obtained during laparoscopy. When CD10 immunohistochemistry results were positive, the corresponding H&E section was reviewed to determine if the initial diagnosis should be revised. Main outcome measure(s) Histologic diagnosis of endometriosis by adjunctive use of CD10 immunohistochemistry in conjunction with H&E-stained specimens. Result(s) In endometrial stroma, CD10 was consistently present. Of the 70 specimens judged negative initially by H&E staining, CD10 staining led to the diagnosis of endometriosis in 11. The addition of CD10 immunohistochemistry detected more positive endometriosis lesions than H&E staining alone (45{\%} vs. 35{\%}). In three women with minimal endometriosis at surgery but initially negative histopathology, CD10 immunohistochemistry changed the histologic diagnosis to endometriosis. Conclusion(s) The adjunctive use of CD10 immunohistochemistry improves diagnostic sensitivity for endometriosis, especially for women with minimal disease.",
keywords = "CD10, diagnostic test, endometriosis, hematoxylin and eosin, immunohistochemistry",
author = "Clariss Potlog-Nahari and Feldman, {Andrew L.} and Pamela Stratton and Koziol, {Deloris E.} and James Segars and Merino, {Maria J.} and Nieman, {Lynnette K.}",
year = "2004",
month = "7",
doi = "10.1016/j.fertnstert.2003.11.059",
language = "English (US)",
volume = "82",
pages = "86--92",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - CD10 immunohistochemical staining enhances the histological detection of endometriosis

AU - Potlog-Nahari, Clariss

AU - Feldman, Andrew L.

AU - Stratton, Pamela

AU - Koziol, Deloris E.

AU - Segars, James

AU - Merino, Maria J.

AU - Nieman, Lynnette K.

PY - 2004/7

Y1 - 2004/7

N2 - Objective To determine whether the use of CD10 immunohistochemistry in addition to hematoxylin and eosin (H&E) staining would increase the sensitivity of surgically suspected endometriosis lesions. Design Retrospective cohort study. Setting Tertiary care government research hospital. Patient(s) Thirty-one women with chronic pelvic pain. Intervention(s) Immunohistochemical analysis for CD10 was performed on 108 possible endometriotic lesions and in the corresponding endometrial biopsy samples obtained during laparoscopy. When CD10 immunohistochemistry results were positive, the corresponding H&E section was reviewed to determine if the initial diagnosis should be revised. Main outcome measure(s) Histologic diagnosis of endometriosis by adjunctive use of CD10 immunohistochemistry in conjunction with H&E-stained specimens. Result(s) In endometrial stroma, CD10 was consistently present. Of the 70 specimens judged negative initially by H&E staining, CD10 staining led to the diagnosis of endometriosis in 11. The addition of CD10 immunohistochemistry detected more positive endometriosis lesions than H&E staining alone (45% vs. 35%). In three women with minimal endometriosis at surgery but initially negative histopathology, CD10 immunohistochemistry changed the histologic diagnosis to endometriosis. Conclusion(s) The adjunctive use of CD10 immunohistochemistry improves diagnostic sensitivity for endometriosis, especially for women with minimal disease.

AB - Objective To determine whether the use of CD10 immunohistochemistry in addition to hematoxylin and eosin (H&E) staining would increase the sensitivity of surgically suspected endometriosis lesions. Design Retrospective cohort study. Setting Tertiary care government research hospital. Patient(s) Thirty-one women with chronic pelvic pain. Intervention(s) Immunohistochemical analysis for CD10 was performed on 108 possible endometriotic lesions and in the corresponding endometrial biopsy samples obtained during laparoscopy. When CD10 immunohistochemistry results were positive, the corresponding H&E section was reviewed to determine if the initial diagnosis should be revised. Main outcome measure(s) Histologic diagnosis of endometriosis by adjunctive use of CD10 immunohistochemistry in conjunction with H&E-stained specimens. Result(s) In endometrial stroma, CD10 was consistently present. Of the 70 specimens judged negative initially by H&E staining, CD10 staining led to the diagnosis of endometriosis in 11. The addition of CD10 immunohistochemistry detected more positive endometriosis lesions than H&E staining alone (45% vs. 35%). In three women with minimal endometriosis at surgery but initially negative histopathology, CD10 immunohistochemistry changed the histologic diagnosis to endometriosis. Conclusion(s) The adjunctive use of CD10 immunohistochemistry improves diagnostic sensitivity for endometriosis, especially for women with minimal disease.

KW - CD10

KW - diagnostic test

KW - endometriosis

KW - hematoxylin and eosin

KW - immunohistochemistry

UR - http://www.scopus.com/inward/record.url?scp=3242786478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3242786478&partnerID=8YFLogxK

U2 - 10.1016/j.fertnstert.2003.11.059

DO - 10.1016/j.fertnstert.2003.11.059

M3 - Article

VL - 82

SP - 86

EP - 92

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 1

ER -