CD10 expression identifies a subset of human perivascular progenitor cells with high proliferation and calcification potentials

Lijun Ding, Bianca Vezzani, Nusrat Khan, Jing Su, Lu Xu, Guijun Yan, Yong Liu, Ruotian Li, Anushri Gaur, Zhenyu Diao, Yali Hu, Zhongzhou Yang, W. Reef Hardy, Aaron W. James, Haixiang Sun, Bruno Péault

Research output: Contribution to journalArticle

Abstract

The tunica adventitia ensheathes arteries and veins and contains presumptive mesenchymal stem cells (MSCs) involved in vascular remodeling. We show here that a subset of human adventitial cells express the CD10/CALLA cell surface metalloprotease. Both CD10+ and CD10 adventitial cells displayed phenotypic features of MSCs when expanded in culture. However, CD10+ adventitial cells exhibited higher proliferation, clonogenic and osteogenic potentials in comparison to their CD10 counterparts. CD10+ adventitial cells increased expression of the cell cycle protein CCND2 via ERK1/2 signaling and osteoblastogenic gene expression via NF-κB signaling. CD10 expression was upregulated in adventitial cells through sonic hedgehog-mediated GLI1 signaling. These results suggest that CD10, which marks rapidly dividing cells in other normal and malignant cell lineages, plays a role in perivascular MSC function and cell fate specification. These findings also point to a role for CD10+ perivascular cells in vascular remodeling and calcification.

Original languageEnglish (US)
Pages (from-to)261-275
Number of pages15
JournalStem Cells
Volume38
Issue number2
DOIs
StatePublished - Feb 1 2020

Keywords

  • CD10
  • GLI1
  • SHH
  • adventitia
  • mesenchymal stem cell
  • osteogenesis
  • vascular calcification

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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  • Cite this

    Ding, L., Vezzani, B., Khan, N., Su, J., Xu, L., Yan, G., Liu, Y., Li, R., Gaur, A., Diao, Z., Hu, Y., Yang, Z., Hardy, W. R., James, A. W., Sun, H., & Péault, B. (2020). CD10 expression identifies a subset of human perivascular progenitor cells with high proliferation and calcification potentials. Stem Cells, 38(2), 261-275. https://doi.org/10.1002/stem.3112