@article{436b182e6d784612ba8f489eae692d30,
title = "CD10 expression identifies a subset of human perivascular progenitor cells with high proliferation and calcification potentials",
abstract = "The tunica adventitia ensheathes arteries and veins and contains presumptive mesenchymal stem cells (MSCs) involved in vascular remodeling. We show here that a subset of human adventitial cells express the CD10/CALLA cell surface metalloprotease. Both CD10+ and CD10− adventitial cells displayed phenotypic features of MSCs when expanded in culture. However, CD10+ adventitial cells exhibited higher proliferation, clonogenic and osteogenic potentials in comparison to their CD10− counterparts. CD10+ adventitial cells increased expression of the cell cycle protein CCND2 via ERK1/2 signaling and osteoblastogenic gene expression via NF-κB signaling. CD10 expression was upregulated in adventitial cells through sonic hedgehog-mediated GLI1 signaling. These results suggest that CD10, which marks rapidly dividing cells in other normal and malignant cell lineages, plays a role in perivascular MSC function and cell fate specification. These findings also point to a role for CD10+ perivascular cells in vascular remodeling and calcification.",
keywords = "CD10, GLI1, SHH, adventitia, mesenchymal stem cell, osteogenesis, vascular calcification",
author = "Lijun Ding and Bianca Vezzani and Nusrat Khan and Jing Su and Lu Xu and Guijun Yan and Yong Liu and Ruotian Li and Anushri Gaur and Zhenyu Diao and Yali Hu and Zhongzhou Yang and Hardy, {W. Reef} and James, {Aaron W.} and Haixiang Sun and Bruno P{\'e}ault",
note = "Funding Information: We thank Dr. Rui Huang for figures organization, Dr. Fiona Rossi for assistance in flow cytometry, Dr. Yue Lin for the help of human tissues collection, Dr. Xiaoqiang Sheng for preparation of manuscript and Dr. Biyun Xu for assistance of statistical analysis. We thank Dr. Ting Wang and Dr. Yanhong Xu for the preparation of human tissue sections. Lijun Ding is supported by grants from National Key Research and Development Program of China (2018YFC1004700), Nature Science Foundation of China (81871128, 81571391) and Nanjing Medical Science Development Project (ZKX16042). Ruotian Li is supported by a grant from Nature Science Foundation of China (81600353). Haixiang Sun is supported by a grant from Jiangsu Province Social Development Project (BE2018602). For this work, Bruno P?ault has been supported by grants from the British Heart Foundation, and BIRAX Regenerative Medicine Initiative. Aaron W. James was supported by the NIH/NIAMS (R01 AR070773, K08 AR068316), NIH/NIDCR (R21 DE027922), Department of Defense (W81XWH-18-1-0121, W81XWH-18-1-0336, W81XWH-18-10613), American Cancer Society (Research Scholar Grant, RSG-18-027-01-CSM), the Maryland Stem Cell Research Foundation, and the Musculoskeletal Transplant Foundation. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Health or Department of Defense. Funding Information: We thank Dr. Rui Huang for figures organization, Dr. Fiona Rossi for assistance in flow cytometry, Dr. Yue Lin for the help of human tissues collection, Dr. Xiaoqiang Sheng for preparation of manuscript and Dr. Biyun Xu for assistance of statistical analysis. We thank Dr. Ting Wang and Dr. Yanhong Xu for the preparation of human tissue sections. Lijun Ding is supported by grants from National Key Research and Development Program of China (2018YFC1004700), Nature Science Foundation of China (81871128, 81571391) and Nanjing Medical Science Development Project (ZKX16042). Ruotian Li is supported by a grant from Nature Science Foundation of China (81600353). Haixiang Sun is supported by a grant from Jiangsu Province Social Development Project (BE2018602). For this work, Bruno P{\'e}ault has been supported by grants from the British Heart Foundation, and BIRAX Regenerative Medicine Initiative. Aaron W. James was supported by the NIH/NIAMS (R01 AR070773, K08 AR068316), NIH/NIDCR (R21 DE027922), Department of Defense (W81XWH‐18‐1‐0121, W81XWH‐18‐1‐0336, W81XWH‐18‐10613), American Cancer Society (Research Scholar Grant, RSG‐18‐027‐01‐CSM), the Maryland Stem Cell Research Foundation, and the Musculoskeletal Transplant Foundation. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Health or Department of Defense. Publisher Copyright: {\textcopyright}AlphaMed Press 2019",
year = "2020",
month = feb,
day = "1",
doi = "10.1002/stem.3112",
language = "English (US)",
volume = "38",
pages = "261--275",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",
number = "2",
}