Abstract
The tunica adventitia ensheathes arteries and veins and contains presumptive mesenchymal stem cells (MSCs) involved in vascular remodeling. We show here that a subset of human adventitial cells express the CD10/CALLA cell surface metalloprotease. Both CD10+ and CD10− adventitial cells displayed phenotypic features of MSCs when expanded in culture. However, CD10+ adventitial cells exhibited higher proliferation, clonogenic and osteogenic potentials in comparison to their CD10− counterparts. CD10+ adventitial cells increased expression of the cell cycle protein CCND2 via ERK1/2 signaling and osteoblastogenic gene expression via NF-κB signaling. CD10 expression was upregulated in adventitial cells through sonic hedgehog-mediated GLI1 signaling. These results suggest that CD10, which marks rapidly dividing cells in other normal and malignant cell lineages, plays a role in perivascular MSC function and cell fate specification. These findings also point to a role for CD10+ perivascular cells in vascular remodeling and calcification.
Original language | English (US) |
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Pages (from-to) | 261-275 |
Number of pages | 15 |
Journal | Stem Cells |
Volume | 38 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1 2020 |
Keywords
- CD10
- GLI1
- SHH
- adventitia
- mesenchymal stem cell
- osteogenesis
- vascular calcification
ASJC Scopus subject areas
- General Medicine