CCR2-mediated recruitment of fibrocytes to the alveolar space after fibrotic injury

Bethany B. Moore, Jill E. Kolodsick, Victor J. Thannickal, Kenneth R Cooke, Thomas A. Moore, Cory Hogaboam, Carol A. Wilke, Galen B. Toews

Research output: Contribution to journalArticle

Abstract

Bone marrow-derived cells are known to play important roles in repair/regeneration of injured tissues, but their roles in pathological fibrosis are less clear. Here, we report a critical role for the chemokine receptor CCR2 in the recruitment and activation of lung fibrocytes (CD45+, CD13+, collagen 1+, CD34-). Lung fibrocytes were isolated in significantly greater numbers from airspaces of fluorescein isothiocyanate-injured CCR2+/+ mice than from CCR2-/- mice. Transplant of CCR2+/+ bone marrow into CCR2-/- recipients restored recruitment of lung flbrocytes and susceptibility to fibrosis. Ex vivo PKH-26-labeled CCR2+/+ lung fibrocytes also migrated to injured air-spaces of CCR2-/- recipients in vivo. Isolated lung fibrocytes expressed CCR2 and migrated to CCL2, and CCL2 stimulated collagen secretion by lung fibrocytes. Fibrocytes could transition into fibroblasts in vitro, and this transition was associated with loss of CCR2 expression and enhanced production of collagen 1. This is the first report describing expression of CCR2 on lung fibrocytes and demonstrating that CCR2 regulates both recruitment and activation of these cells after respiratory injury.

Original languageEnglish (US)
Pages (from-to)675-684
Number of pages10
JournalAmerican Journal of Pathology
Volume166
Issue number3
StatePublished - Mar 2005
Externally publishedYes

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Lung
Wounds and Injuries
Collagen
Fibrosis
Chemokine Receptors
Fluorescein
Bone Marrow Cells
Regeneration
Fibroblasts
Bone Marrow
Air
Transplants

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Moore, B. B., Kolodsick, J. E., Thannickal, V. J., Cooke, K. R., Moore, T. A., Hogaboam, C., ... Toews, G. B. (2005). CCR2-mediated recruitment of fibrocytes to the alveolar space after fibrotic injury. American Journal of Pathology, 166(3), 675-684.

CCR2-mediated recruitment of fibrocytes to the alveolar space after fibrotic injury. / Moore, Bethany B.; Kolodsick, Jill E.; Thannickal, Victor J.; Cooke, Kenneth R; Moore, Thomas A.; Hogaboam, Cory; Wilke, Carol A.; Toews, Galen B.

In: American Journal of Pathology, Vol. 166, No. 3, 03.2005, p. 675-684.

Research output: Contribution to journalArticle

Moore, BB, Kolodsick, JE, Thannickal, VJ, Cooke, KR, Moore, TA, Hogaboam, C, Wilke, CA & Toews, GB 2005, 'CCR2-mediated recruitment of fibrocytes to the alveolar space after fibrotic injury', American Journal of Pathology, vol. 166, no. 3, pp. 675-684.
Moore BB, Kolodsick JE, Thannickal VJ, Cooke KR, Moore TA, Hogaboam C et al. CCR2-mediated recruitment of fibrocytes to the alveolar space after fibrotic injury. American Journal of Pathology. 2005 Mar;166(3):675-684.
Moore, Bethany B. ; Kolodsick, Jill E. ; Thannickal, Victor J. ; Cooke, Kenneth R ; Moore, Thomas A. ; Hogaboam, Cory ; Wilke, Carol A. ; Toews, Galen B. / CCR2-mediated recruitment of fibrocytes to the alveolar space after fibrotic injury. In: American Journal of Pathology. 2005 ; Vol. 166, No. 3. pp. 675-684.
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