CBP Regulates Recruitment and Release of Promoter-Proximal RNA Polymerase II

Ann Boija, Dig Bijay Mahat, Aman Zare, Per Henrik Holmqvist, Philge Philip, David J Meyers, Philip A. Cole, John T. Lis, Per Stenberg, Mattias Mannervik

Research output: Contribution to journalArticle

Abstract

Transcription activation involves RNA polymerase II (Pol II) recruitment and release from the promoter into productive elongation, but how specific chromatin regulators control these steps is unclear. Here, we identify a novel activity of the histone acetyltransferase p300/CREB-binding protein (CBP) in regulating promoter-proximal paused Pol II. We find that Drosophila CBP inhibition results in "dribbling" of Pol II from the pause site to positions further downstream but impedes transcription through the +1 nucleosome genome-wide. Promoters strongly occupied by CBP and GAGA factor have high levels of paused Pol II, a unique chromatin signature, and are highly expressed regardless of cell type. Interestingly, CBP activity is rate limiting for Pol II recruitment to these highly paused promoters through an interaction with TFIIB but for transit into elongation by histone acetylation at other genes. Thus, CBP directly stimulates both Pol II recruitment and the ability to traverse the first nucleosome, thereby promoting transcription of most genes. CBP and p300 have known functions at transcriptional enhancers. Boija et al. identify a regulatory role for the Drosophila p300/CBP homolog also at promoters. Drosophila CBP maintains RNA Pol II at the promoter-proximal pause site, recruits Pol II through an interaction with TFIIB, and facilitates transcription through the +1 nucleosome.

Original languageEnglish (US)
JournalMolecular Cell
DOIs
StateAccepted/In press - 2017

Fingerprint

CREB-Binding Protein
RNA Polymerase II
Nucleosomes
Transcription Factor TFIIB
Drosophila Proteins
Chromatin
Histone Acetyltransferases
Acetylation
Histones
Transcriptional Activation
Genes
Drosophila
Genome

Keywords

  • CBP
  • Drosophila
  • GAGA-factor
  • Histone acetyltransferase
  • P300
  • Polymerase pausing
  • TFIIB
  • Transcription
  • Trithorax-like

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Boija, A., Mahat, D. B., Zare, A., Holmqvist, P. H., Philip, P., Meyers, D. J., ... Mannervik, M. (Accepted/In press). CBP Regulates Recruitment and Release of Promoter-Proximal RNA Polymerase II. Molecular Cell. https://doi.org/10.1016/j.molcel.2017.09.031

CBP Regulates Recruitment and Release of Promoter-Proximal RNA Polymerase II. / Boija, Ann; Mahat, Dig Bijay; Zare, Aman; Holmqvist, Per Henrik; Philip, Philge; Meyers, David J; Cole, Philip A.; Lis, John T.; Stenberg, Per; Mannervik, Mattias.

In: Molecular Cell, 2017.

Research output: Contribution to journalArticle

Boija, A, Mahat, DB, Zare, A, Holmqvist, PH, Philip, P, Meyers, DJ, Cole, PA, Lis, JT, Stenberg, P & Mannervik, M 2017, 'CBP Regulates Recruitment and Release of Promoter-Proximal RNA Polymerase II', Molecular Cell. https://doi.org/10.1016/j.molcel.2017.09.031
Boija, Ann ; Mahat, Dig Bijay ; Zare, Aman ; Holmqvist, Per Henrik ; Philip, Philge ; Meyers, David J ; Cole, Philip A. ; Lis, John T. ; Stenberg, Per ; Mannervik, Mattias. / CBP Regulates Recruitment and Release of Promoter-Proximal RNA Polymerase II. In: Molecular Cell. 2017.
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