CBFβ-SMMHC, expressed in M4eo acute myeloid leukemia, reduces p53 induction and slows apoptosis in hematopoietic cells exposed to DNA-damaging agents

Martin Britos-Bray, Manuel Ramirez, Wangsen Cao, Xinping Wang, P. Paul Liu, Curt I. Civin, Alan D. Friedman

Research output: Contribution to journalArticlepeer-review

Abstract

CBFβ-SMMHC is expressed in M4Eo acute myeloid leukemia (AML) as a result of inv(16), but how it contributes to leukemogenesis is unknown. p53 mutations are rare in de novo AML, but they are common in many malignancies. Expression of CBFβ-SMMHC in Ba/F3 cells reduced p53 induction in response to ionizing radiation or etoposide 3- to 4-fold. However, p53 induction was normal in Ba/F3 cells expressing a CBFβ-SMMHC variant that does not interfere with DNA binding by CBF, indicating that a CBF genetic target regulates p53 induction. The p53 gene may be regulated by CBF, because p53 mRNA levels were reduced by CBFβ-SMMHC. Reduced p53 induction was not caused by slowed cell proliferation, a consequence of CBFβ-SMMHC expression, because p53 was induced similarly in control cultures and in cultures propagated in 10-fold less interleukin-3 (IL-3). CBFβ-SMMHC did not slow apoptosis resulting from IL-3 withdrawal, where p53 induction is minimal, but slowed apoptosis in Ba/F3 cells exposed to 10 Gy of ionizing radiation or 3 to 8 μg/mL etoposide, providing 2-fold protection at 6 or 18 hours. Inhibition of apoptosis was temporary, because all the cells exposed to these doses ultimately died, and clonal survival assays performed using 0.04 μg/mL etoposide did not show protection by CBFβ-SMMHC. p21 levels were increased in cells subjected to DNA damage, regardless of CBFβ-SMMHC expression and attenuated p53 induction. Bcl-2, bcl-x(L), bcl-x(S), and bax levels were unaffected by CBFβ-SMMHC. Attenuated p53 induction may contribute to leukemogenesis by CBFβ-SMMHC by slowing apoptosis via a p21-independent mechanism.

Original languageEnglish (US)
Pages (from-to)4344-4352
Number of pages9
JournalBlood
Volume92
Issue number11
DOIs
StatePublished - Dec 1 1998

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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