Cationic gelatin as a gene carrier

K. E. Brown, J. Bathon, C. H. Huang, R. Dalal, K. W. Leong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cationic gelatin (CG) has been evaluated as a non-viral vector for cell transfection. CG is synthesized by modifying gelatin with hexanediamine. Its complexation with psv-8-gal plasmid caused an electrophoretic mobility shift of the plasmid. CG/DNA complexes are optimized in terms of transfection efficiency in CHODUK XB1 and COS 7 cell lines. Maximal gene expression for both cell types occurred in serum free medium with chloroquine at cationic gelatin/DNA ratios of about two and seven. Compared to DEAF dextran, polysine and Lipofectamine, CG is the most efficient in transfecting COS 7 cells. In a dye reduction cytotoxicity assay, CG caused

Original languageEnglish (US)
Title of host publicationMaterials Research Society Symposium - Proceedings
PublisherMaterials Research Society
Pages61-66
Number of pages6
Volume394
StatePublished - 1995
EventProceedings of the 1995 MRS Spring Meeting - San Francisco, CA, USA
Duration: Apr 17 1995Apr 21 1995

Other

OtherProceedings of the 1995 MRS Spring Meeting
CitySan Francisco, CA, USA
Period4/17/954/21/95

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials

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    Brown, K. E., Bathon, J., Huang, C. H., Dalal, R., & Leong, K. W. (1995). Cationic gelatin as a gene carrier. In Materials Research Society Symposium - Proceedings (Vol. 394, pp. 61-66). Materials Research Society.