Cathepsin D of rabbit skin: An immunoenzymic study

G. S. Lazarus, J. T. Dingle

Research output: Contribution to journalArticlepeer-review


Cathepsin D, one of the major lysosomal proteinases, has been identified in rabbit skin. A line of complete immunologic identity between crude skin extracts and pure rabbit liver cathepsin D was found in immunodiffusion studies using sheep antirabbit cathepsin D antiserum. The addition of sheep antiserum against rabbit cathepsin D to skin extracts resulted in the precipitation of 85% of the hemoglobin degrading activity measured at pH 3.2. Selective removal of the enzyme from the skin extracts by precipitation with antibody revealed that cathepsin D was responsible for degradation of skin protein at acid pH. Cathepsin D may play a major role in both extracellular and intracellular catabolism in skin.

Original languageEnglish (US)
Pages (from-to)61-66
Number of pages6
JournalJournal of Investigative Dermatology
Issue number2
StatePublished - 1974
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology


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