Cathepsin D of rabbit skin: An immunoenzymic study

Gerald Sylvan Lazarus, J. T. Dingle

Research output: Contribution to journalArticle

Abstract

Cathepsin D, one of the major lysosomal proteinases, has been identified in rabbit skin. A line of complete immunologic identity between crude skin extracts and pure rabbit liver cathepsin D was found in immunodiffusion studies using sheep antirabbit cathepsin D antiserum. The addition of sheep antiserum against rabbit cathepsin D to skin extracts resulted in the precipitation of 85% of the hemoglobin degrading activity measured at pH 3.2. Selective removal of the enzyme from the skin extracts by precipitation with antibody revealed that cathepsin D was responsible for degradation of skin protein at acid pH. Cathepsin D may play a major role in both extracellular and intracellular catabolism in skin.

Original languageEnglish (US)
Pages (from-to)61-66
Number of pages6
JournalJournal of Investigative Dermatology
Volume62
Issue number2
StatePublished - 1974
Externally publishedYes

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Cathepsin D
Skin
Rabbits
Immune Sera
Sheep
Immunodiffusion
Complex Mixtures
Liver
Proteolysis
Hemoglobins
Peptide Hydrolases
Degradation
Acids
Antibodies
Enzymes
Proteins

ASJC Scopus subject areas

  • Dermatology

Cite this

Cathepsin D of rabbit skin : An immunoenzymic study. / Lazarus, Gerald Sylvan; Dingle, J. T.

In: Journal of Investigative Dermatology, Vol. 62, No. 2, 1974, p. 61-66.

Research output: Contribution to journalArticle

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