Catching HIV 'in the act' with 3D electron microscopy

Lesley A. Earl; Jeffrey D. Lifson; Sriram Subramaniam

doi:10.1016/j.tim.2013.06.004

Catching HIV 'in the act' with 3D electron microscopy

Lesley A. Earl, Jeffrey D. Lifson, Sriram Subramaniam

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The development of a safe, effective vaccine to prevent HIV infection is a key step for controlling the disease on a global scale. However, many aspects of HIV biology make vaccine design problematic, including the sequence diversity and structural variability of the surface envelope glycoproteins and the poor accessibility of neutralization-sensitive epitopes on the virus. In this review, we discuss recent progress in understanding HIV in a structural context using emerging tools in 3D electron microscopy, and outline how some of these advances could be important for a better understanding of mechanisms of viral entry and for vaccine design.

Original language	English (US)
Pages (from-to)	397-404
Number of pages	8
Journal	Trends in Microbiology
Volume	21
Issue number	8
DOIs	https://doi.org/10.1016/j.tim.2013.06.004
State	Published - Aug 2013
Externally published	Yes

Keywords

Cryo-electron tomography
Focused ion beam scanning electron microscopy (FIB-SEM)
Ion abrasion scanning electron microscopy (IA-SEM)
Vaccine design
Virus-cell interaction

ASJC Scopus subject areas

Infectious Diseases
Microbiology (medical)
Microbiology
Virology

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10.1016/j.tim.2013.06.004

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title = "Catching HIV 'in the act' with 3D electron microscopy",

abstract = "The development of a safe, effective vaccine to prevent HIV infection is a key step for controlling the disease on a global scale. However, many aspects of HIV biology make vaccine design problematic, including the sequence diversity and structural variability of the surface envelope glycoproteins and the poor accessibility of neutralization-sensitive epitopes on the virus. In this review, we discuss recent progress in understanding HIV in a structural context using emerging tools in 3D electron microscopy, and outline how some of these advances could be important for a better understanding of mechanisms of viral entry and for vaccine design.",

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year = "2013",

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AU - Lifson, Jeffrey D.

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AB - The development of a safe, effective vaccine to prevent HIV infection is a key step for controlling the disease on a global scale. However, many aspects of HIV biology make vaccine design problematic, including the sequence diversity and structural variability of the surface envelope glycoproteins and the poor accessibility of neutralization-sensitive epitopes on the virus. In this review, we discuss recent progress in understanding HIV in a structural context using emerging tools in 3D electron microscopy, and outline how some of these advances could be important for a better understanding of mechanisms of viral entry and for vaccine design.

KW - Cryo-electron tomography

KW - Focused ion beam scanning electron microscopy (FIB-SEM)

KW - Ion abrasion scanning electron microscopy (IA-SEM)

KW - Vaccine design

KW - Virus-cell interaction

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Catching HIV 'in the act' with 3D electron microscopy

Abstract

Keywords

ASJC Scopus subject areas

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