Catalytic mechanisms and regulation of protein kinases

Zhihong Wang, Philip A. Cole

Research output: Contribution to journalArticle

Abstract

Protein kinases transfer a phosphoryl group from ATP onto target proteins and play a critical role in signal transduction and other cellular processes. Here, we review the kinase kinetic and chemical mechanisms and their application in understanding kinase structure and function. Aberrant kinase activity has been implicated in many human diseases, in particular cancer. We highlight applications of technologies and concepts derived from kinase mechanistic studies that have helped illuminate how kinases are regulated and contribute to pathophysiology.

Original languageEnglish (US)
Pages (from-to)1-21
Number of pages21
JournalMethods in Enzymology
Volume548
Issue numberC
DOIs
StatePublished - 2014

Fingerprint

Protein Kinases
Phosphotransferases
Signal transduction
Signal Transduction
Adenosine Triphosphate
Technology
Kinetics
Neoplasms
Proteins

Keywords

  • B-Raf
  • Bisubstrate analog
  • Chemical rescue
  • EGFR
  • Erlotinib
  • Inhibitor
  • Lapatinib
  • Src
  • Transition state

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Catalytic mechanisms and regulation of protein kinases. / Wang, Zhihong; Cole, Philip A.

In: Methods in Enzymology, Vol. 548, No. C, 2014, p. 1-21.

Research output: Contribution to journalArticle

Wang, Zhihong ; Cole, Philip A. / Catalytic mechanisms and regulation of protein kinases. In: Methods in Enzymology. 2014 ; Vol. 548, No. C. pp. 1-21.
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