TY - JOUR
T1 - Catabolism of polyamines in the rat. Polyamines and their non-α-amino acid metabolites
AU - Watkins, Paul A.
AU - Yost, David A.
AU - Chang, Anne W.
AU - Mekalanos, John J.
AU - Moss, Joel
N1 - Funding Information:
The authors thank the Central Animal Laboratory (head: Mr. H. Dikken) for their technical assistance with the handling of rats. Drs. H. Verwey and B.G. Wolthers are gratefully acknowledged for their useful discussions and advice, and Mrs. J.E. Koornstra-Rijskamp for her dedicated assistance with the manuscript. This work was supported in part by grant no GUKC 83-16 (Dr. G.A. Van den Berg) from the Koningin Wilhelmina Fonds (the Netherlands Cancer Foundation).
PY - 1984
Y1 - 1984
N2 - The metabolic fate of stable isotopically labeled polyamines was investigated after their first and second intraperitoneal injection in rats. Using gas chromatographic and mass fragmentographic analyses of acid-hydrolyzed 24-h urines, some aspects of the polyamine metabolism could be elucidated. After the injections with hexadeutero-1,3-diaminopropane, only labeled 1,3-diaminopropane was recovered from the urine samples. The rat injected with tetradeuteroputrescine excreted labeled putrescine, γ-amino-n-butyric acid, 2-hydroxyputrescine and spermidine, while the urine samples of the rat after the injections with tetradeuterocadaverine contained labeled cadaverine and δ-aminovaleric acid. The injections of hexadeuterospermidine led to the appearance of labeled spermidine, isoputreanine, putreanine, N-(2-carboxyethyl)-4-amino-n-butyric acid, putrescine, γ-amino-n-butyric acid, 1,3-diaminopropane, β-alanine and spermine. After the injections with octadeuterospermine, labeled spermine N-(3-aminopropyl)-N'-(2-carboxyethyl)-1,4-diaminobutane, N,N'-bis(2-carboxyethyl)-1,4-diaminobutane, spermidine, isoputreanine, putreanine, N-(2-carboxyethyl)-4-amino-n-butyric acid, putrescine, 1,3-diaminopropane, β-alanine, 2-hydroxyputrescine and possibly γ-amino-n-butyric acid were recovered. Clear differences between the metabolism after the first and second injection were noted for putrescine, spermidine and spermine, which is suggestive for enzyme induction and/or the existence of salvage pathways.
AB - The metabolic fate of stable isotopically labeled polyamines was investigated after their first and second intraperitoneal injection in rats. Using gas chromatographic and mass fragmentographic analyses of acid-hydrolyzed 24-h urines, some aspects of the polyamine metabolism could be elucidated. After the injections with hexadeutero-1,3-diaminopropane, only labeled 1,3-diaminopropane was recovered from the urine samples. The rat injected with tetradeuteroputrescine excreted labeled putrescine, γ-amino-n-butyric acid, 2-hydroxyputrescine and spermidine, while the urine samples of the rat after the injections with tetradeuterocadaverine contained labeled cadaverine and δ-aminovaleric acid. The injections of hexadeuterospermidine led to the appearance of labeled spermidine, isoputreanine, putreanine, N-(2-carboxyethyl)-4-amino-n-butyric acid, putrescine, γ-amino-n-butyric acid, 1,3-diaminopropane, β-alanine and spermine. After the injections with octadeuterospermine, labeled spermine N-(3-aminopropyl)-N'-(2-carboxyethyl)-1,4-diaminobutane, N,N'-bis(2-carboxyethyl)-1,4-diaminobutane, spermidine, isoputreanine, putreanine, N-(2-carboxyethyl)-4-amino-n-butyric acid, putrescine, 1,3-diaminopropane, β-alanine, 2-hydroxyputrescine and possibly γ-amino-n-butyric acid were recovered. Clear differences between the metabolism after the first and second injection were noted for putrescine, spermidine and spermine, which is suggestive for enzyme induction and/or the existence of salvage pathways.
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U2 - 10.1016/0304-4165(85)90221-1
DO - 10.1016/0304-4165(85)90221-1
M3 - Article
C2 - 6498214
AN - SCOPUS:84879282698
SN - 0304-4165
VL - 802
SP - 175
EP - 187
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 2
ER -