[Ca2+]i independent mitogenesis in cultured human fibroblasts revealed by single cell microfluorimetry

Frank C. Praeger, Leslie Reinlib, Mark Donowitz, Barbara A. Gilchrest

Research output: Contribution to journalArticle

Abstract

A transient rise in intracellular free Ca2+ concentration ([Ca2+]i) has been implicated in mitogenic induction of cell division. Individual human foreskin fibroblasts in confluent cultures examined with the Ca2+ indicator Fura-2 and a fluorescence microscope-imaging system had a basal [Ca2+]i which varied markedly from cell-to-cell. A transient serum-induced rise in [Ca2+]i was demonstrated the magnitude of which was directly correlated with the basal [Ca2+]i level. In contrast to serum-induced increase in [Ca2+]i, exposure to an elevated level of extracellular Ca2+, which is at least equally mitogenic for fibroblasts, did not alter the basal [Ca2+]i of single subconfluent cells or confluent cells. Elevated extracellular Ca2+ does not exert its mitogenicity via a transient rise in [Ca2+]i.

Original languageEnglish (US)
Pages (from-to)862-870
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume159
Issue number2
DOIs
StatePublished - Mar 15 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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