[Ca2+](i) Oscillation frequency regulates agonist-stimulated NF-κB transcriptional activity

Qinghua Hu, Shailesh Deshpande, Kaikobad Irani, Roy C. Ziegelstein

Research output: Contribution to journalArticle

Abstract

In nonexcitable cells, stimulation by high agonist concentrations typically produces a biphasic increase in cytosolic Ca2+ ([Ca2+](i)). This response is characterized by a transient initial increase because of intracellular Ca2+ release followed by a sustained elevation which varies in amplitude depending on the nature of the stimulus. In contrast, low-level stimulation often evokes oscillatory changes in [Ca2+](i). The specific information provided by repetitive [Ca2+](i) spikes appears to be encoded in the frequency rather than in the amplitude of [Ca2+](i) oscillations. The specific, membrane-permeable inositol 1,4,5-trisphosphate (Ins-1,4,5-P3) receptor blocker Xestospongin C (XeC, 2-20 μM) was used to affect [Ca2+](i) signaling in human aortic endothelial cells (HAEC) during an established response to low-level (1 μM) histamine stimulation. XeC produced a dose-dependent decrease in the frequency of [Ca2+](i) oscillations during histamine stimulation without affecting oscillation amplitude. Histamine stimulated a 14-fold increase in NF-κB-chloramphenicol acetyltransferase reporter gene activity that was dose-dependently decreased by XeC. Thus, during low-level agonist stimulation, [Ca2+](i) oscillation frequency regulates nuclear transcription in HAEC.

Original languageEnglish (US)
Pages (from-to)33995-33998
Number of pages4
JournalJournal of Biological Chemistry
Volume274
Issue number48
DOIs
StatePublished - Nov 26 1999

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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