Castor is required for Hedgehog-dependent cell-fate specification and follicle stem cell maintenance in Drosophila oogenesis

Yu Chiuan Chang, Anna C.C. Jang, Cheng Han Lin, Denise J. Montell

Research output: Contribution to journalArticle

Abstract

Asymmetric division of stem cells results in both self-renewal and differentiation of daughters. Understanding the molecules and mechanisms that govern differentiation of specific cell types from adult tissue stem cells is a major challenge in developmental biology and regenerative medicine. Drosophila follicle stem cells (FSCs) represent an excellent model system to study adult stem cell behavior; however, the earliest stages of follicle cell differentiation remain largely mysterious. Here we identify Castor (Cas) as a nuclear protein that is expressed in FSCs and early follicle cell precursors and then is restricted to differentiated polar and stalk cells once egg chambers form. Cas is required for FSC maintenance and polar and stalk cell fate specification. Eyes absent (Eya) is excluded from polar and stalk cells and represses their fate by inhibiting Cas expression. Hedgehog signaling is essential to repress Eya to allow Cas expression in polar and stalk cells. Finally, we show that the complementary patterns of Cas and Eya reveal the gradual differentiation of polar and stalk precursor cells at the earliest stages of their development. Our studies provide a marker for cell fates in this model and insight into the molecular and cellular mechanisms by which FSC progeny diverge into distinct fates.

Original languageEnglish (US)
Pages (from-to)E1734-E1742
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number19
DOIs
StatePublished - May 7 2013

Keywords

  • Germarium
  • Polar cell
  • Polar stalk precursor

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Castor is required for Hedgehog-dependent cell-fate specification and follicle stem cell maintenance in Drosophila oogenesis'. Together they form a unique fingerprint.

  • Cite this