Caspofungin reduces the incidence of fungal contamination in cell culture

Luis R. Martinez, Patricia Ntiamoah, Arturo Casadevall, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

Abstract

Fungal contamination is a major problem in cell culture, and the antifungal compounds currently in use can affect cultured cells. Echinocandins are antifungal drugs that inhibit fungal cell wall synthesis by targeting an enzyme that has no counterpart in mammalian cells. We evaluated whether the echinocandin caspofungin affected the growth or morphology of six murine cell lines (a macrophage-like cell line (J774.16) and five hybridoma lines), or primary human endothelial cells. The antifungal did not influence cellular characteristics at concentrations less than 512 μg/ml, while effectively reducing the incidence of fungal contamination. Also, caspofungin did not affect the production of antibody by hybridoma cells, or alter the cytokine production of J774.16 cells, although modest increases in IL-4 and IFN-γ occurred upon LPS stimulation. Hence, echinocandins appear to be relatively non-toxic, and protect against fungal contamination in cell culture.

Original languageEnglish (US)
Pages (from-to)279-286
Number of pages8
JournalMycopathologia
Volume164
Issue number6
DOIs
StatePublished - Dec 1 2007
Externally publishedYes

Keywords

  • Amphotericin B
  • Caspofungin
  • Cell culture
  • Echinocandins
  • Fungi

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science
  • veterinary (miscalleneous)

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