Case-control study of vitamin D, dickkopf homolog 1 (DKK1) gene methylation, VDR gene polymorphism and the risk of colon adenoma in African Americans

Hassan Ashktorab, Bijou Nguza, Mehrnaz Fatemi, Mehdi Nouraie, Duane T. Smoot, Alejandro A. Schäffer, Sonia S. Kupfer, Carlos A. Camargo, Hassan Brim

Research output: Contribution to journalArticle

Abstract

Background: There are sparse data on genetic, epigenetic and vitamin D exposure in African Americans (AA) with colon polyp. Consequently, we evaluated serum 25(OH) D levels, vitamin D receptor (VDR) polymorphisms and the methylation status of the tumor suppressor gene dickkopf homolog 1 (DKK1) as risk factors for colon polyp in this population. Methods: The case-control study consisted of 93 patients with colon polyp (cases) and 187 healthy individuals (controls) at Howard University Hospital. Serum levels of 25(OH)D (including D3, D2, and total) were measured by liquid chromatography-mass spectrometry. DNA analysis focused on 49 single nucleotide polymorphisms (SNPs) in the VDR gene. Promoter methylation analysis of DKK1 was also performed. The resulting data were processed in unadjusted and multivariable logistic regression analyses. Results: Cases and controls differed in vitamin D status (D 33 (3 and colon polyp in AAs. VDR SNPs and DKK1 methylation were not associated with colon polyp. Vitamin D levels may in part explain the higher incidence of polyp in AAs.

Original languageEnglish (US)
Article numbere25314
JournalPLoS One
Volume6
Issue number10
DOIs
StatePublished - Oct 13 2011
Externally publishedYes

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Calcitriol Receptors
Methylation
adenoma
African Americans
Polyps
vitamin D
case-control studies
Polymorphism
Vitamin D
Adenoma
methylation
colon
Case-Control Studies
Colon
Genes
genetic polymorphism
receptors
Nucleotides
genes
Liquid chromatography

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Case-control study of vitamin D, dickkopf homolog 1 (DKK1) gene methylation, VDR gene polymorphism and the risk of colon adenoma in African Americans. / Ashktorab, Hassan; Nguza, Bijou; Fatemi, Mehrnaz; Nouraie, Mehdi; Smoot, Duane T.; Schäffer, Alejandro A.; Kupfer, Sonia S.; Camargo, Carlos A.; Brim, Hassan.

In: PLoS One, Vol. 6, No. 10, e25314, 13.10.2011.

Research output: Contribution to journalArticle

Ashktorab, H, Nguza, B, Fatemi, M, Nouraie, M, Smoot, DT, Schäffer, AA, Kupfer, SS, Camargo, CA & Brim, H 2011, 'Case-control study of vitamin D, dickkopf homolog 1 (DKK1) gene methylation, VDR gene polymorphism and the risk of colon adenoma in African Americans', PLoS One, vol. 6, no. 10, e25314. https://doi.org/10.1371/journal.pone.0025314
Ashktorab, Hassan ; Nguza, Bijou ; Fatemi, Mehrnaz ; Nouraie, Mehdi ; Smoot, Duane T. ; Schäffer, Alejandro A. ; Kupfer, Sonia S. ; Camargo, Carlos A. ; Brim, Hassan. / Case-control study of vitamin D, dickkopf homolog 1 (DKK1) gene methylation, VDR gene polymorphism and the risk of colon adenoma in African Americans. In: PLoS One. 2011 ; Vol. 6, No. 10.
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