Cas9 deactivation with photocleavable guide RNAs

Roger S. Zou; Yang Liu; Bin Wu; Taekjip Ha

doi:10.1016/j.molcel.2021.02.007

Cas9 deactivation with photocleavable guide RNAs

Roger S. Zou, Yang Liu, Bin Wu, Taekjip Ha

School of Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Zou et al. developed light-mediated control over Cas9 and base editor deactivation by introducing a photocleavable moiety into guide RNA. Deactivation occurs within seconds and approaches completeness. This modification natively enhanced specificity. Timed deactivation facilitated studies of DNA repair by synchronizing the termination of DNA damage within a cell population.

Original language	English (US)
Pages (from-to)	1553-1565.e8
Journal	Molecular cell
Volume	81
Issue number	7
DOIs	https://doi.org/10.1016/j.molcel.2021.02.007
State	Published - Apr 1 2021

Keywords

CRISPR
Cas9
DNA repair
deactivation
genome editing
specificity

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.molcel.2021.02.007

Cite this

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title = "Cas9 deactivation with photocleavable guide RNAs",

abstract = "Zou et al. developed light-mediated control over Cas9 and base editor deactivation by introducing a photocleavable moiety into guide RNA. Deactivation occurs within seconds and approaches completeness. This modification natively enhanced specificity. Timed deactivation facilitated studies of DNA repair by synchronizing the termination of DNA damage within a cell population.",

keywords = "CRISPR, Cas9, DNA repair, deactivation, genome editing, specificity",

author = "Zou, {Roger S.} and Yang Liu and Bin Wu and Taekjip Ha",

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AU - Liu, Yang

AU - Wu, Bin

AU - Ha, Taekjip

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N2 - Zou et al. developed light-mediated control over Cas9 and base editor deactivation by introducing a photocleavable moiety into guide RNA. Deactivation occurs within seconds and approaches completeness. This modification natively enhanced specificity. Timed deactivation facilitated studies of DNA repair by synchronizing the termination of DNA damage within a cell population.

AB - Zou et al. developed light-mediated control over Cas9 and base editor deactivation by introducing a photocleavable moiety into guide RNA. Deactivation occurs within seconds and approaches completeness. This modification natively enhanced specificity. Timed deactivation facilitated studies of DNA repair by synchronizing the termination of DNA damage within a cell population.

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KW - DNA repair

KW - deactivation

KW - genome editing

KW - specificity

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ER -

Cas9 deactivation with photocleavable guide RNAs

Abstract

Keywords

ASJC Scopus subject areas

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