Cas adaptor proteins organize the retinal ganglion cell layer downstream of integrin signaling

Martin M. Riccomagno, Lu O. Sun, Colleen M. Brady, Konstantina Alexandropoulos, Sachiko Seo, Mineo Kurokawa, Alex L Kolodkin

Research output: Contribution to journalArticle

Abstract

Stratification of retinal neuronal cell bodies and lamination of their processes provide a scaffold upon which neural circuits can be built. However, the molecular mechanisms that direct retinal ganglion cells (RGCs) to resolve into a single-cell retinal ganglioncell layer (GCL) are not well understood. The extracellular matrix protein laminin conveys spatial information that instructs the migration, process outgrowth, and reorganization of GCL cells. Here, we show that the β1-Integrin laminin receptor is required for RGC positioning and reorganization into a single-cell GCL layer. β1-Integrin signaling within migrating GCL cells requires Cas signaling-adaptor proteins, and in the absence of β1-Integrin or Cas function retinal neurons form ectopic cell clusters beyond the inner-limiting membrane (ILM), phenocopying laminin mutants. These data reveal an essential role for Cas adaptor proteins in β1-Integrin-mediated signaling events critical for the formation of the single-cell GCL in the mammalian retina.

Original languageEnglish (US)
Pages (from-to)779-786
Number of pages8
JournalNeuron
Volume81
Issue number4
DOIs
StatePublished - Feb 19 2014

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Retinal Ganglion Cells
Integrins
Proteins
Laminin
Laminin Receptors
Retinal Neurons
Extracellular Matrix Proteins
Retina
Membranes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Riccomagno, M. M., Sun, L. O., Brady, C. M., Alexandropoulos, K., Seo, S., Kurokawa, M., & Kolodkin, A. L. (2014). Cas adaptor proteins organize the retinal ganglion cell layer downstream of integrin signaling. Neuron, 81(4), 779-786. https://doi.org/10.1016/j.neuron.2014.01.036

Cas adaptor proteins organize the retinal ganglion cell layer downstream of integrin signaling. / Riccomagno, Martin M.; Sun, Lu O.; Brady, Colleen M.; Alexandropoulos, Konstantina; Seo, Sachiko; Kurokawa, Mineo; Kolodkin, Alex L.

In: Neuron, Vol. 81, No. 4, 19.02.2014, p. 779-786.

Research output: Contribution to journalArticle

Riccomagno, MM, Sun, LO, Brady, CM, Alexandropoulos, K, Seo, S, Kurokawa, M & Kolodkin, AL 2014, 'Cas adaptor proteins organize the retinal ganglion cell layer downstream of integrin signaling', Neuron, vol. 81, no. 4, pp. 779-786. https://doi.org/10.1016/j.neuron.2014.01.036
Riccomagno MM, Sun LO, Brady CM, Alexandropoulos K, Seo S, Kurokawa M et al. Cas adaptor proteins organize the retinal ganglion cell layer downstream of integrin signaling. Neuron. 2014 Feb 19;81(4):779-786. https://doi.org/10.1016/j.neuron.2014.01.036
Riccomagno, Martin M. ; Sun, Lu O. ; Brady, Colleen M. ; Alexandropoulos, Konstantina ; Seo, Sachiko ; Kurokawa, Mineo ; Kolodkin, Alex L. / Cas adaptor proteins organize the retinal ganglion cell layer downstream of integrin signaling. In: Neuron. 2014 ; Vol. 81, No. 4. pp. 779-786.
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